| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4199036 | B | Induction of ULK1 phosphorylation at Ser317 in human SH-SY5Y cells at 5 uM after 6 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 2. | ALA4199037 | B | Induction of ULK1 phosphorylation at Ser757 in human SH-SY5Y cells at 5 uM after 36 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 3. | ALA4199038 | B | Activation of ULK1 in human SH-SY5Y cells assessed as mAtg13 phosphorylation at Ser318 at 5 uM after 12 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 4. | ALA4199039 | B | Activation of ULK1 in human SH-SY5Y cells assessed as mAtg13 phosphorylation at Ser318 at 5 uM after 24 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 5. | ALA4199040 | B | Activation of ULK1 in human SH-SY5Y cells assessed as mAtg13 phosphorylation at Ser318 at 5 uM after 36 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 6. | ALA4199041 | B | Induction of human recombinant N-terminal GST tagged-ULK1 (1 to 649 residues) kinase activity expressed in baculovirus infected insect Sf9 cells at 100 nM using MBP as substrate after 60 mins by ADP-glo assay relative to control | Homo sapiens | 84 | ALA4196093 | cell-based format | Scientific Literature | |
| 7. | ALA4199042 | B | Induction of ULK1-mediated autophagy in human SH-SY5Y cells at 1 uM after 6 hrs by MDC-staining based flow cytometric analysis relative to control | Homo sapiens | 84 | ALA4196093 | cell-based format | Scientific Literature | |
| 8. | ALA4199043 | B | Induction of human recombinant C-terminal His tagged-AMPK alpha1/beta1/gamma2 kinase activity expressed in baculovirus infected insect Sf9 cells using SAMStide as substrate after 60 mins by ADP-glo assay | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 9. | ALA4199044 | B | Induction of eEF2K (unknown origin) kinase activity expressed in Escherichia coli using MH-1 as substrate after 2 hrs by ADP-glo assay relative to control | Homo sapiens | 1 | ALA4196093 | assay format | Scientific Literature | |
| 10. | ALA4199045 | B | Induction of recombinant FLAG tagged-ULK1 K50A mutant (unknown origin) kinase activity expressed in HEK293T cells using MBP as substrate after 60 mins by ADP-glo assay | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 11. | ALA4199046 | B | Induction of recombinant FLAG tagged-ULK1 R18A mutant (unknown origin) kinase activity expressed in HEK293T cells using MBP as substrate after 60 mins by ADP-glo assay | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 12. | ALA4199047 | B | Induction of recombinant FLAG tagged-ULK1 N86A mutant (unknown origin) kinase activity expressed in HEK293T cells using MBP as substrate after 60 mins by ADP-glo assay | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 13. | ALA4199048 | B | Induction of recombinant FLAG tagged-ULK1 Y89A mutant (unknown origin) kinase activity expressed in HEK293T cells using MBP as substrate after 60 mins by ADP-glo assay | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 14. | ALA4199049 | B | Induction of recombinant FLAG tagged-ULK1 K50A mutant (unknown origin) kinase activity expressed in HEK293T cells assessed as mAtg13 phosphorylation at Ser318 co-transfected with GST-tagged mAtg13 for 20 mins measured by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 15. | ALA4199050 | B | Induction of recombinant FLAG tagged-ULK1 R18A mutant (unknown origin) kinase activity expressed in HEK293T cells assessed as mAtg13 phosphorylation at Ser318 co-transfected with GST-tagged mAtg13 for 20 mins measured by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 16. | ALA4199051 | B | Induction of recombinant FLAG tagged-ULK1 N86A mutant (unknown origin) kinase activity expressed in HEK293T cells assessed as mAtg13 phosphorylation at Ser318 co-transfected with GST-tagged mAtg13 for 20 mins measured by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 17. | ALA4199052 | B | Induction of recombinant FLAG tagged-ULK1 Y89A mutant (unknown origin) kinase activity expressed in HEK293T cells assessed as mAtg13 phosphorylation at Ser318 co-transfected with GST-tagged mAtg13 for 20 mins measured by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 18. | ALA4199053 | B | Induction of recombinant FLAG tagged-ULK1 K50A mutant (unknown origin) kinase activity expressed in HEK293T cells assessed as mAtg13 phosphorylation at Ser318 using GST-tagged mAtg13 measured after 20 mins by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 19. | ALA4199054 | B | Induction of recombinant FLAG tagged-ULK1 R18A mutant (unknown origin) kinase activity expressed in HEK293T cells assessed as mAtg13 phosphorylation at Ser318 using GST-tagged mAtg13 measured after 20 mins by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature | |
| 20. | ALA4199055 | B | Induction of recombinant FLAG tagged-ULK1 N86A mutant (unknown origin) kinase activity expressed in HEK293T cells assessed as mAtg13 phosphorylation at Ser318 using GST-tagged mAtg13 measured after 20 mins by Western blot analysis relative to control | Homo sapiens | 1 | ALA4196093 | cell-based format | Scientific Literature |
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