# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4178563 | B | Ratio of Kinact to Ki for recombinant His6-tagged PAD1 (unknown origin) preincubated for 10 mins followed by BAEE substrate addition measured after 15 mins by COLDER assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
2. | ALA4178564 | B | Ratio of Kinact to Ki for recombinant human PAD2 expressed in Escherichia coli BL21(DE3)pLysS cells preincubated for 10 mins followed by BAEE substrate addition measured after 15 mins by COLDER assay | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
3. | ALA4178565 | B | Ratio of Kinact to Ki for recombinant His6-tagged PAD3 (unknown origin) preincubated for 10 mins followed by BAA substrate addition measured after 15 mins by COLDER assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
4. | ALA4178566 | B | Ratio of Kinact to Ki for recombinant full length N-terminal GST-tagged human PAD4 expressed in Escherichia coli Rosetta cells preincubated for 10 mins followed by BAEE substrate addition measured after 15 mins by COLDER assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
5. | ALA4178567 | B | Binding affinity to human PAD2 expressed in HEK293T cells assessed as protein occupancy preincubated for 15 mins followed by ionomycin addition measured after 1 hr in presence of CaCl2 by RIBFA probe based SDS-PAGE analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
6. | ALA4178569 | B | Binding affinity to human PAD2 expressed in HEK293T cells assessed as decrease in histone H3 citrulination incubated for 3 hrs in presence of ionomycin/CaCl2 by SDS-PAGE analysis | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
7. | ALA4185519 | F | Cytotoxicity against human U2OS cells after 72 hrs by XTT assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
8. | ALA5035492 | F | Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay | Homo sapiens | 2 | cell-based format | Scientific Literature |