# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4198189 | Binding | Inhibition of N-terminal GST-tagged full length recombinant human PDE5A1 catalytic domain expressed in Baculovirus infected Sf9 insect cells using FAM-cGMP as substrate after 1 hr by fluorescence polarization assay | Homo sapiens | 17 | ALA4196066 | cell-based format | Scientific Literature | |
2. | ALA4198190 | Binding | Selectivity ratio of IC50 for N-terminal GST-tagged full length recombinant human PDE1A to IC50 for N-terminal GST-tagged full length recombinant human PDE5A1 catalytic domain | Homo sapiens | 16 | ALA4196066 | assay format | Scientific Literature | |
3. | ALA4198191 | Binding | Selectivity ratio of IC50 for N-terminal FLAG-tagged human PDE2A1 (2 to 941 residues) to IC50 for N-terminal GST-tagged full length recombinant human PDE5A1 catalytic domain | Homo sapiens | 16 | ALA4196066 | assay format | Scientific Literature | |
4. | ALA4198192 | Binding | Selectivity ratio of IC50 for N-terminal GST-tagged recombinant human PDE3B (592 end residues) to IC50 for N-terminal GST-tagged full length recombinant human PDE5A1 catalytic domain | Homo sapiens | 16 | ALA4196066 | assay format | Scientific Literature | |
5. | ALA4198193 | Binding | Selectivity ratio of IC50 for N-terminal GST-tagged full length recombinant human PDE4A1A to IC50 for N-terminal GST-tagged full length recombinant human PDE5A1 catalytic domain | Homo sapiens | 16 | ALA4196066 | assay format | Scientific Literature | |
6. | ALA4198194 | Binding | Selectivity ratio of IC50 for N-terminal GST-tagged full length recombinant human PDE6C to IC50 for N-terminal GST-tagged full length recombinant human PDE5A1 catalytic domain | Homo sapiens | 18 | ALA4196066 | assay format | Scientific Literature | |
7. | ALA4198195 | Binding | Selectivity ratio of IC50 for N-terminal GST-tagged full length recombinant human PDE11A to IC50 for N-terminal GST-tagged full length recombinant human PDE5A1 catalytic domain | Homo sapiens | 18 | ALA4196066 | assay format | Scientific Literature | |
8. | ALA4198220 | Functional | Vasorelaxant activity in norepinephrine-pretreated New Zealand rabbit thoracic aorta assessed as increase in aortic relaxation at 10 uM measured after 15 mins relative to control | Oryctolagus cuniculus | 2 | ALA4196066 | tissue-based format | Scientific Literature | |
9. | ALA4198222 | Binding | Inhibition of N-terminal GST-tagged full length recombinant human PDE6C expressed in Baculovirus infected Sf9 insect cells using FAM-cAMP as substrate after 1 hr by fluorescence polarization assay | Homo sapiens | 8 | ALA4196066 | cell-based format | Scientific Literature | |
10. | ALA4198223 | Binding | Inhibition of N-terminal GST-tagged full length recombinant human PDE11A expressed in Baculovirus infected Sf9 insect cells using FAM-cAMP as substrate after 1 hr by fluorescence polarization assay | Homo sapiens | 8 | ALA4196066 | cell-based format | Scientific Literature | |
11. | ALA4198226 | Binding | Inhibition of N-terminal GST-tagged full length recombinant human PDE1A expressed in Baculovirus infected Sf9 insect cells at 250 uM using FAM-cAMP as substrate after 1 hr by fluorescence polarization assay relative to control | Homo sapiens | 1 | ALA4196066 | cell-based format | Scientific Literature | |
12. | ALA4198234 | Binding | Inhibition of N-terminal FLAG-tagged human PDE2A1 (2 to 941 residues) expressed in Baculovirus infected Sf9 insect cells at 40 uM using FAM-cAMP as substrate after 1 hr by fluorescence polarization assay relative to control | Homo sapiens | 1 | ALA4196066 | cell-based format | Scientific Literature | |
13. | ALA4198241 | Binding | Inhibition of N-terminal GST-tagged recombinant human PDE3B (592 end residues) expressed in Baculovirus infected Sf9 insect cells at 250 uM using FAM-cAMP as substrate after 1 hr by fluorescence polarization assay relative to control | Homo sapiens | 1 | ALA4196066 | cell-based format | Scientific Literature | |
14. | ALA4198247 | Binding | Inhibition of N-terminal GST-tagged full length recombinant human PDE4A1A expressed in Baculovirus infected Sf9 insect cells at 50 uM using FAM-cAMP as substrate after 1 hr by fluorescence polarization assay relative to control | Homo sapiens | 1 | ALA4196066 | cell-based format | Scientific Literature |