Inhibition of ALR2 in Wistar rat eye lens assessed as reduction in NADPH consumption preincubated for 1 min followed by D,L-glyceraldehyde addition measured after 4 mins by spectrophotometric analysis
Inhibition of ALR1 in Wistar rat kidney assessed as reduction in NADPH consumption preincubated for 1 min followed by substrate addition measured after 4 mins by spectrophotometric analysis
Inhibition of ALR2 in Wistar rat eye lens assessed as Vmax for substrate per 1 mg protein at 10 nM preincubated for 1 min followed by D,L-glyceraldehyde addition measured after 4 mins by spectrophotometric analysis (Rvb = 11.05 +/- 2.41 10'4 OD/S)
Inhibition of ALR2 in Wistar rat eye lens assessed as Km for substrate at 10 nM preincubated for 1 min followed by D,L-glyceraldehyde addition measured after 4 mins by spectrophotometric analysis (Rvb = 0.116 +/- 0.025 nM)
Uncompetitive inhibition of ALR2 in Wistar rat eye lens assessed as reduction in NADPH consumption preincubated for 1 min followed by D,L-glyceraldehyde addition measured after 4 mins by spectrophotometric analysis
Inhibition of ALR2 in Wistar rat lens assessed as reduction in glucose induced-sorbitol accumulation by measuring sorbitol level at 0.1 uM preincubated for 30 mins followed by glucose addition measured after 3 hrs by resazurin dye based fluorescence assay (Rvb = 803.6 +/- 104.6 nmol/g)
Inhibition of ALR2 in Wistar rat lens assessed as reduction in glucose induced-sorbitol accumulation by measuring sorbitol level at 1 uM preincubated for 30 mins followed by glucose addition measured after 3 hrs by resazurin dye based fluorescence assay (Rvb = 803.6 +/- 104.6 nmol/g)
Inhibition of ALR2 in Wistar rat lens assessed as reduction in glucose induced-sorbitol accumulation by measuring sorbitol level at 10 uM preincubated for 30 mins followed by glucose addition measured after 3 hrs by resazurin dye based fluorescence assay (Rvb = 803.6 +/- 104.6 nmol/g)
Inhibition of ALR2 in Wistar rat lens assessed as reduction in glucose induced-sorbitol accumulation by measuring sorbitol level at 100 uM preincubated for 30 mins followed by glucose addition measured after 3 hrs by resazurin dye based fluorescence assay (Rvb = 803.6 +/- 104.6 nmol/g)
Inhibition of ALR2 in Wistar rat lens assessed as reduction in glucose induced-sorbitol accumulation by measuring sorbitol level preincubated for 30 mins followed by glucose addition measured after 3 hrs by resazurin dye based fluorescence assay
Inhibition of ALR2 in Wistar rat eye lens assessed as Vmax for substrate per 1 mg protein at 20 nM preincubated for 1 min followed by D,L-glyceraldehyde addition measured after 4 mins by spectrophotometric analysis (Rvb = 11.05 +/- 2.41 10'4 OD/S)
Inhibition of ALR2 in Wistar rat eye lens assessed as Km for substrate at 20 nM preincubated for 1 min followed by D,L-glyceraldehyde addition measured after 4 mins by spectrophotometric analysis (Rvb = 0.116 +/- 0.025 nM)