# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4194326 | B | Inhibition of Hedgehog signaling pathway in mouse C3H10T1/2 cells assessed as reduction in purmorphamine-induced increase in alkaline phosphatase level using CDP-star as substrate pretreated for 1 hr followed by purmorphamine addition measured after 5 days by luminescence assay | Mus musculus | 27 | ALA4190359 | cell-based format | Scientific Literature | |
2. | ALA4194328 | A | Cytotoxicity against mouse C3H10T1/2 cells assessed as cell viability by CellTiter-Glo assay | Mus musculus | 26 | ALA4190359 | cell-based format | Scientific Literature | |
3. | ALA4194329 | B | Inhibition of Hedgehog signaling in mouse Shh Light2 cells assessed as reduction in purmorphamine-induced Gli mediated transcriptional activity pretreated for 1 hr followed by purmorphamine addition measured after 48 hrs by luciferase reporter gene assay | Mus musculus | 14 | ALA4190359 | cell-based format | Scientific Literature | |
4. | ALA4194331 | A | Cytotoxicity against mouse Shh Light2 cells assessed as cell viability by CellTiter Glo assay | Mus musculus | 13 | ALA4190359 | cell-based format | Scientific Literature | |
5. | ALA4194333 | B | Inhibition of recombinant human C-terminal His6-tagged alkaline phosphate activity (Leu18 to Ser502 residues) using CDP-star as substrate pretreated for 30 mins followed by substrate addition measured after 60 mins by luminescence assay | Homo sapiens | 27 | ALA4190359 | single protein format | Scientific Literature |