| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4178339 | B | Inhibition of recombinant human Cdc7 (1 to 574 residues)/human N-terminal GST-tagged DBF4 (1 to 674 residues) expressed in baculovirus expression system using His-tagged MCM2 as substrate preincubated for 10 mins followed by ATP addition by HTRF assay | Homo sapiens | 26 | ALA4177537 | assay format | Scientific Literature | |
| 2. | ALA4178340 | B | Inhibition of recombinant human Cdk2 (1 to 298 residues)/human N-terminal GST-tagged cyclinE (1 to 410 residues) expressed in baculovirus expression system using histone H1 as substrate after 90 mins in presence of ATP by kinase-glo assay | Homo sapiens | 23 | ALA4177537 | assay format | Scientific Literature | |
| 3. | ALA4178341 | B | Inhibition of recombinant human N-terminal GST-tagged ROCK1 (1 to 477 residues) expressed in baculovirus expression system using biotin-STK substrate-2 as substrate preincubated for 5 mins followed by ATP addition after 2 hrs by TR-FRET assay | Homo sapiens | 24 | ALA4177537 | assay format | Scientific Literature | |
| 4. | ALA4178342 | B | Selectivity ratio of IC50 for recombinant human Cdk2 (1 to 298 residues)/human N-terminal GST-tagged cyclinE (1 to 410 residues to IC50 for recombinant human Cdc7 (1 to 574 residues)/human N-terminal GST-tagged DBF4 (1 to 674 residues) | Homo sapiens | 23 | ALA4177537 | assay format | Scientific Literature | |
| 5. | ALA4178343 | B | Selectivity ratio of IC50 for human N-terminal GST-tagged ROCK1 (1 to 477 residues) to IC50 for recombinant human Cdc7 (1 to 574 residues)/human N-terminal GST-tagged DBF4 (1 to 674 residues) | Homo sapiens | 23 | ALA4177537 | assay format | Scientific Literature | |
| 6. | ALA4178344 | B | Inhibition of Cdc7 in human HeLa cells assessed as reduction in MCM2 phosphorylation at ser40 residues after 7 hrs by Western blot analysis | Homo sapiens | 4 | ALA4177537 | cell-based format | Scientific Literature | |
| 7. | ALA4178345 | F | Cytotoxicity against human COLO205 cells after 3 days by CellTiter-Glo luminescent cell viability assay | Homo sapiens | 4 | ALA4177537 | cell-based format | Scientific Literature | |
| 8. | ALA4178346 | P | Solubility of the compound | 4 | ALA4177537 | small-molecule physicochemical format | Scientific Literature |
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