Inhibition of Enterovirus 71 Shenzhen/120F1/09 capsid infected in human RD cells assessed as reduction in virus-induced cell death after 72 hrs by CCK-8 assay
Apparent half-life of the terminal disposition phase in Sprague-Dawley rat at 5 mg/kg, po administered as single dose measured upto 24 hrs by LC-MS/MS analysis
Inhibition of capsid protein VP1 in Enterovirus 71 (FY)-Luc infected in human RD cells assessed as reduction in virus-induced cell death preincubated for 24 hrs followed by fluorescein substrate addition and measured after 5 mins by chemiluminescence analysis
Subacute toxicity in PND4 Sprague-Dawley rat assessed as animal survival at 30 to 50 mg/kg/day, po administered via gavage for 14 days and measured for 28 days
Subacute toxicity in PND4 Sprague-Dawley rat assessed as irreversible opaqueness of lens at 30 to 50 mg/kg/day, po administered via gavage for 14 days and measured for 28 days
Subacute toxicity in PND14 Sprague-Dawley rat assessed as development of opaque lens at 100 mg/kg/day, po administered via gavage for 14 days and measured for 28 days
Subacute toxicity in PND14 Sprague-Dawley rat assessed as quadriplegia at 100 mg/kg/day, po administered via gavage for 14 days and measured for 28 days