#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4188950	Binding	Inhibition of recombinant Escherichia coli N-terminal His-tagged rhomboid protease GlpG expressed in Escherichia coli C43(DE3) cells at 250 uM using gurken as substrate preincubated for 30 mins followed by substrate addition measured after 90 mins by SDS-PAGE analysis	Escherichia coli	12	ALA4184297	cell-based format	Scientific Literature
2.	ALA4188952	Binding	Inhibition of recombinant Escherichia coli N-terminal His-tagged rhomboid protease GlpG expressed in Escherichia coli C43(DE3) cells using KSp76 as substrate incubated for 1 hr followed by substrate addition by fluorescence assay	Escherichia coli	1	ALA4184297	cell-based format	Scientific Literature
3.	ALA4188953	Binding	Inhibition of bovine alpha-chymotrypsin at 250 uM using N-Succinyl-Ala-AlaPro-Phe-p-nitroanilide as substrate incubated for 30 mins followed by substrate addition measured over 5 mins by spectroscopic method	Bos taurus	2	ALA4184297	single protein format	Scientific Literature
4.	ALA4188954	Binding	Inhibition of bovine pancreas trypsin at 10 uM using Z-Gly-Gly-Argaminomethylcoumarin as substrate preincubated for 30 mins followed by substrate addition measured at 1 min time interval for 5 mins by fluorescence assay	Bos taurus	4	ALA4184297	assay format	Scientific Literature
5.	ALA4188955	Binding	Inhibition of human neutrophil elastase at 10 uM using N-Methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilid as substrate preincubated for 20 mins followed by substrate addition measured at 1 min time interval for 5 mins by fluorescence assay	Homo sapiens	4	ALA4184297	single protein format	Scientific Literature
6.	ALA4188956	Binding	Inhibition of bovine pancreas trypsin assessed as remaining activity at 50 uM using Z-Gly-Gly-Argaminomethylcoumarin as substrate preincubated for 30 mins followed by substrate addition measured at 1 min time interval for 5 mins by fluorescence assay relative to control	Bos taurus	1	ALA4184297	assay format	Scientific Literature
7.	ALA4188958	Binding	Inhibition of human neutrophil cathepsin G at 10 uM using N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide as substrate preincubated for 30 mins followed by substrate addition measured at 1 min time interval for 5 mins by fluorescence assay	Homo sapiens	3	ALA4184297	single protein format	Scientific Literature
8.	ALA4188959	Binding	Binding affinity to wild type recombinant Escherichia coli rhomboid protease GlpG expressed in Escherichia coli C43(DE3) cells by electrospray mass spectrometric analysis	Escherichia coli	3	ALA4184297	cell-based format	Scientific Literature
9.	ALA4188960	Binding	Binding affinity to recombinant Escherichia coli rhomboid protease GlpG S201T mutant expressed in Escherichia coli C43(DE3) cells by electrospray mass spectrometric analysis	Escherichia coli	1	ALA4184297	cell-based format	Scientific Literature
10.	ALA4188961	Binding	Binding affinity to recombinant Escherichia coli rhomboid protease GlpG H254A mutant expressed in Escherichia coli C43(DE3) cells by electrospray mass spectrometric analysis	Escherichia coli	1	ALA4184297	cell-based format	Scientific Literature
11.	ALA4188962	Binding	Binding affinity to recombinant Escherichia coli rhomboid protease GlpG His150 mutant expressed in Escherichia coli C43(DE3) cells by electrospray mass spectrometric analysis	Escherichia coli	1	ALA4184297	cell-based format	Scientific Literature
12.	ALA4188963	Binding	Reversible inhibition of recombinant Escherichia coli N-terminal His-tagged rhomboid protease GlpG expressed in Escherichia coli C43(DE3) cells assessed as recovery of enzyme activity at 50 uM using KSp76 as substrate preincubated for 1 hr followed by substrate addition measured over 120 mins by rapid dilution based fluorescence assay	Escherichia coli	1	ALA4184297	cell-based format	Scientific Literature