#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4200947	Binding	Inhibition of mouse His-tagged IDE (42 to 1019 residues) using fluorophore/quencher-tagged RPPGFSAFK(Dnp)-OH peptide as substrate preincubated for 10 mins followed by substrate addition measured over 5 mins by TR-FRET assay	Mus musculus	14	ALA4196173	single protein format	Scientific Literature
2.	ALA4200948	Binding	Binding affinity to IDE E111Q mutant (unknown origin) using N-terminal FITC-labeled compound after 2 hrs in absence of light at 298 K by fluorescence polarization assay	Homo sapiens	1	ALA4196173	single protein format	Scientific Literature
3.	ALA4200949	Binding	Selectivity ratio of IC50 for ECE-1 (unknown origin) to IC50 for mouse His-tagged IDE (42 to 1019 residues)		1	ALA4196173	assay format	Scientific Literature
4.	ALA4200950	Binding	Selectivity ratio of IC50 for ACE (unknown origin) to IC50 for mouse His-tagged IDE (42 to 1019 residues)		1	ALA4196173	assay format	Scientific Literature
5.	ALA4200951	Binding	Inhibition of IDE (unknown origin) assessed as potentiation of insulin-induced AKT phosphorylation in rat skeletal muscle cells at 100 uM preincubated for 10 mins followed insulin addition measured by Western blot analysis	Homo sapiens	1	ALA4196173	cell-based format	Scientific Literature
6.	ALA4200952	Binding	Selectivity ratio of IC50 for NEP (unknown origin) to IC50 for mouse His-tagged IDE (42 to 1019 residues)		1	ALA4196173	assay format	Scientific Literature
7.	ALA4200953	ADME	Drug uptake in human HeLa cells at 5 uM using FITC-labeled compound after 2 hrs at 310 K by DAPI-staining based confocal microscopy	Homo sapiens	4	ALA4196173	cell-based format	Scientific Literature
8.	ALA4200954	ADME	Drug uptake in human HeLa cells at 5 uM using FITC-labeled compound after 2 hrs at 310 K by trypan blue-fluorescence based FACS analysis	Homo sapiens	4	ALA4196173	cell-based format	Scientific Literature
9.	ALA4200955	ADME	Drug uptake in human HeLa cells at 5 uM using FITC-labeled compound after 2 hrs at 310 K by LC-MS analysis	Homo sapiens	1	ALA4196173	cell-based format	Scientific Literature
10.	ALA4200956	ADME	Cytotoxicity against human HeLa cells assessed as reduction in cell viability at <300 uM after 24 hrs at 310 K by MTT assay	Homo sapiens	2	ALA4196173	cell-based format	Scientific Literature
11.	ALA4200957	ADME	Hemolytic activity against mouse RBC at 300 uM after 1 hr at 310 K	Mus musculus	3	ALA4196173	cell-based format	Scientific Literature
12.	ALA4200958	ADME	Metabolic stability in 25% mouse serum assessed as compound remaining at 250 uM up to 24 hrs by LC-MS analysis	Mus musculus	2	ALA4196173	cell-free format	Scientific Literature
13.	ALA4200959	Binding	Inhibition of IDE (unknown origin) assessed as potentiation of insulin-induced AKT phosphorylation in rat skeletal muscle cells at 100 uM preincubated up to 12 hrs followed by insulin addition and measured after 10 mins by Western blot analysis	Homo sapiens	1	ALA4196173	cell-based format	Scientific Literature
14.	ALA4200960	Binding	Inhibition of IDE (unknown origin)-mediated FITC-insulin degradation in rat skeletal muscle cells at 100 uM after 10 mins at 310 K by DAPI-staining based fluorescent inverted microscopic imaging analysis	Homo sapiens	1	ALA4196173	cell-based format	Scientific Literature
15.	ALA4200961	Toxicity	Toxicity in lean C57BL/6J mouse assessed as behavioral changes at 80 mg/kg, ip	Mus musculus	1	ALA4196173	organism-based format	Scientific Literature
16.	ALA4200962	Toxicity	Toxicity in lean C57BL/6J mouse assessed as body weight change at 80 mg/kg, ip after 3 days	Mus musculus	1	ALA4196173	organism-based format	Scientific Literature
17.	ALA4200963	Functional	Anti-diabetic activity in DIO C57BL/6J mouse model assessed as reduction in glucose levels at 80 mg/kg, ip administered 30 min prior to glucose stimulation measured up to 120 mins post glucose injection by GTT	Mus musculus	1	ALA4196173	organism-based format	Scientific Literature
18.	ALA4200964	ADME	Effect on glucose levels in lean C57BL/6J mouse at 80 mg/kg, ip administered 30 min prior to glucose stimulation measured up to 120 min post glucose injection by GTT	Mus musculus	1	ALA4196173	organism-based format	Scientific Literature
19.	ALA4200965	Functional	Hypoglycemic activity in lean C57BL/6J mouse at 80 mg/kg, ip administered 30 min prior to insulin injection measured up to 75 mins post insulin injection by ITT	Mus musculus	1	ALA4196173	organism-based format	Scientific Literature
20.	ALA4200966	ADME	Drug uptake in brain parenchyma of DIO C57BL/6J mouse at 80 mg/kg, iv using FITC-labeled compound by two-photon microscopic analysis	Mus musculus	1	ALA4196173	organism-based format	Scientific Literature