# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4201827 | B | Inhibition of His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 pre-incubated for 30 mins before N-Boc-FSR-AMC substrate addition and measured after 30 mins | Homo sapiens | 43 | ALA4196210 | assay format | Scientific Literature | |
2. | ALA4201828 | B | Inhibition of trypsin (unknown origin) pre-incubated for 30 mins before N-Boc-FSR-AMC substrate addition and measured after 30 mins | Homo sapiens | 41 | ALA4196210 | protein format | Scientific Literature | |
3. | ALA4201840 | B | Binding affinity to His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 assessed as acyl-enzyme complex stability half life pre-incubated for 1 hr followed by excess compound removal by ESI-MS analysis | Homo sapiens | 3 | ALA4196210 | assay format | Scientific Literature | |
4. | ALA4201853 | P | Stability in DMEM supplemented with 10% (v/v) fetal bovine serum at 10 uM at 37 degC assessed as half life by HPLC-MS method | 7 | ALA4196210 | small-molecule physicochemical format | Scientific Literature |