# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4198900 | A | Inhibition of full length human recombinant C-terminal FLAG/His-tagged HDAC1 expressed in baculovirus infected sf9 cells using fluorogenic HDAC substrate 3 after 30 mins by fluorescence assay | Homo sapiens | 27 | ALA4196089 | cell-based format | Scientific Literature | |
2. | ALA4198901 | A | Inhibition of full length human recombinant C-terminal His-tagged HDAC2 expressed in baculovirus infected sf9 cells using fluorogenic HDAC substrate 3 after 30 mins by fluorescence assay | Homo sapiens | 26 | ALA4196089 | cell-based format | Scientific Literature | |
3. | ALA4198902 | A | Inhibition of full length human recombinant C-terminal His-tagged HDAC3/N-terminal GST-tagged NCOR2 (95 to 489 residues) expressed in baculovirus infected sf9 cells using fluorogenic HDAC substrate 3 after 30 mins by fluorescence assay | Homo sapiens | 5 | ALA4196089 | cell-based format | Scientific Literature | |
4. | ALA4198903 | B | Inhibition of full length human recombinant N-terminal GST-tagged HDAC6 expressed in baculovirus infected sf9 cells using fluorogenic HDAC substrate 3 after 30 mins by fluorescence assay | Homo sapiens | 26 | ALA4196089 | cell-based format | Scientific Literature | |
5. | ALA4198905 | A | Inhibition of full length human recombinant C-terminal FLAG/His-tagged HDAC1 expressed in baculovirus infected sf9 cells using fluorogenic HDAC substrate 3 after 4 hrs fluorescence assay | Homo sapiens | 3 | ALA4196089 | cell-based format | Scientific Literature | |
6. | ALA4198909 | A | Inhibition of full length human recombinant C-terminal FLAG/His-tagged HDAC1 expressed in baculovirus infected sf9 cells assessed residence time preincubated fopr 2 hrs followed by dilution and subsequent fluorogenic HDAC substrate 3 addition measured at 2 mins time interval by fluorescence based jump dilution assay | Homo sapiens | 3 | ALA4196089 | cell-based format | Scientific Literature | |
7. | ALA4198913 | B | Inhibition of full length human recombinant N-terminal GST-tagged PDE5A1 expressed in baculovirus infected sf9 cells using cGMP as substrate after 30 mins by fluorescence assay | Homo sapiens | 25 | ALA4196089 | cell-based format | Scientific Literature | |
8. | ALA4198914 | A | Cytotoxicity against human THLE2 cells after 72 hrs by vialight cell proliferation assay | Homo sapiens | 17 | ALA4196089 | cell-based format | Scientific Literature | |
9. | ALA4198915 | B | Inhibition of HDAC6 in human SH-SY5Y cells assessed as induction of tubulin K-40 acetylation level at 500 nM after 2 hrs by Western blot analysis relative to control | Homo sapiens | 17 | ALA4196089 | cell-based format | Scientific Literature | |
10. | ALA4198917 | B | Inhibition of HDAC6 in human SH-SY5Y cells assessed as induction of tubulin K-40 acetylation level at 100 nM after 2 hrs by Western blot analysis relative to control | Homo sapiens | 17 | ALA4196089 | cell-based format | Scientific Literature | |
11. | ALA4198918 | B | Inhibition of HDAC6 in human SH-SY5Y cells assessed as induction of tubulin K-40 acetylation level at 1000 nM after 2 hrs by Western blot analysis relative to control | Homo sapiens | 17 | ALA4196089 | cell-based format | Scientific Literature | |
12. | ALA4198919 | A | Inhibition of class-1 HDAC in human SH-SY5Y cells assessed as induction of AcH3K9 level at 100 nM after 2 hrs by Western blot analysis relative to control | Homo sapiens | 17 | ALA4196089 | cell-based format | Scientific Literature | |
13. | ALA4198920 | A | Inhibition of class-1 HDAC in human SH-SY5Y cells assessed as induction of AcH3K9 level at 500 nM after 2 hrs by Western blot analysis relative to control | Homo sapiens | 17 | ALA4196089 | cell-based format | Scientific Literature | |
14. | ALA4198921 | A | Inhibition of class-1 HDAC in human SH-SY5Y cells assessed as induction of AcH3K9 level at 1000 nM after 2 hrs by Western blot analysis relative to control | Homo sapiens | 17 | ALA4196089 | cell-based format | Scientific Literature | |
15. | ALA4198922 | B | Inhibition of HDAC6 in human SH-SY5Y cells assessed as induction of tubulin K-40 acetylation level after 2 hrs by Western blot analysis | Homo sapiens | 12 | ALA4196089 | cell-based format | Scientific Literature | |
16. | ALA4198923 | B | Inhibition of PDE5 in human SH-SY5Y cells assessed as induction of phosphorylated CREB level at 500 nM after 30 mins by Western blot analysis relative to control | Homo sapiens | 17 | ALA4196089 | cell-based format | Scientific Literature | |
17. | ALA4198924 | B | Inhibition of PDE5 in human SH-SY5Y cells assessed as induction of phosphorylated CREB level at 500 nM after 2 hrs by Western blot analysis relative to control | Homo sapiens | 17 | ALA4196089 | cell-based format | Scientific Literature | |
18. | ALA4198925 | A | Effective permeability of the compound at 50 uM after 18 hrs by UV spectroscopy based PAMPA | 16 | ALA4196089 | assay format | Scientific Literature | ||
19. | ALA4198927 | F | Selectivity ratio of compound effect for inhibition of HDAC6 in human SH-SY5Y cells assessed as induction of tubulin K-40 acetylation level to compound effect for inhibition of class-1 HDAC in human SH-SY5Y cells assessed as induction of AcH3K9 level | Homo sapiens | 1 | ALA4196089 | cell-based format | Scientific Literature | |
20. | ALA4198928 | A | Inhibition of class-1 HDAC in human SH-SY5Y cells assessed as induction of AcH3K9 level at 160 nM after 2 hrs by AlphaLISA assay | Homo sapiens | 1 | ALA4196089 | cell-based format | Scientific Literature |