#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4187427	Binding	Inhibition of C-terminal 6-His tagged recombinant Clostridium difficile toxin B catalytic fragment (Met1 to Leu543 residues) assessed as reduction in glucosyltransferase domain UDP-glucose hydrolysis activity using UDP-glucose and ADP Alexa633 tracer incubated fore 3 hrs by fluorescence polarization assay	Clostridioides difficile	45	ALA4184209	single protein format	Scientific Literature
2.	ALA4187428	Binding	Inhibition of Clostridium difficile toxin B transfected in CHO cells assessed as reduction in caspase 3/7 activation pre-incubated for 1 hr before TcdB addition by luminescence based assay1	Clostridioides difficile	12	ALA4184209	cell-based format	Scientific Literature
3.	ALA4187429	Binding	Inhibition of Clostridium difficile toxin A transfected in CHO cells assessed as reduction in caspase 3/7 activation pre-incubated for 1 hr before TcdA addition by luminescence based assay1	Clostridioides difficile	12	ALA4184209	cell-based format	Scientific Literature
4.	ALA4187430	ADME	Stability in human liver microsomes assessed as parent compound remaining level incubated for 15 mins	Homo sapiens	12	ALA4184209	microsome format	Scientific Literature
5.	ALA4187431	ADME	Stability in mouse liver microsomes assessed as parent compound remaining level incubated for 15 mins	Mus musculus	12	ALA4184209	microsome format	Scientific Literature
6.	ALA4187432	Functional	Effect on growth of Clostridium difficile at 0.5 to 100 uM incubated for 24 hrs under anaerobic conditions by spectrophotometry	Clostridioides difficile	45	ALA4184209	organism-based format	Scientific Literature
7.	ALA4187433	ADME	Effect on growth of Lactobacillus reuteri at 0.5 to 100 uM incubated for 24 hrs under anaerobic conditions by spectrophotometry	Limosilactobacillus reuteri	45	ALA4184209	organism-based format	Scientific Literature
8.	ALA4187434	ADME	Effect on growth of Bifidobacterium longum at 0.5 to 100 uM incubated for 24 hrs under anaerobic conditions by spectrophotometry	Bifidobacterium longum	45	ALA4184209	organism-based format	Scientific Literature
9.	ALA4187435	ADME	Effect on growth of Bacteroides fragilis at 0.5 to 100 uM incubated for 48 hrs under anaerobic conditions by spectrophotometry	Bacteroides fragilis	45	ALA4184209	organism-based format	Scientific Literature
10.	ALA4187436	ADME	Clearance in CD1 mouse at 1 mg/kg, iv	Mus musculus	2	ALA4184209	organism-based format	Scientific Literature
11.	ALA4187437	ADME	Elimination half life in CD1 mouse at 1 mg/kg, iv	Mus musculus	2	ALA4184209	organism-based format	Scientific Literature
12.	ALA4187438	ADME	Apparent volume of distribution in CD1 mouse at 1 mg/kg, iv	Mus musculus	2	ALA4184209	organism-based format	Scientific Literature
13.	ALA4187439	ADME	AUC (0 to 24 hrs) in CD1 mouse at 1 mg/kg, iv	Mus musculus	2	ALA4184209	organism-based format	Scientific Literature
14.	ALA4187440	ADME	AUC (0 to 24 hrs) in CD1 mouse at 10 mg/kg, po	Mus musculus	2	ALA4184209	organism-based format	Scientific Literature
15.	ALA4187441	ADME	Cmax in CD1 mouse at 1 mg/kg, iv	Mus musculus	2	ALA4184209	organism-based format	Scientific Literature
16.	ALA4187442	ADME	Cmax in CD1 mouse at 10 mg/kg, po	Mus musculus	2	ALA4184209	organism-based format	Scientific Literature
17.	ALA4187443	ADME	Tmax in CD1 mouse at 10 mg/kg, po	Mus musculus	2	ALA4184209	organism-based format	Scientific Literature
18.	ALA4187444	ADME	Stability in mouse plasma assessed as parent compound remaining measured after 15 mins post dose	Mus musculus	1	ALA4184209	cell-free format	Scientific Literature
19.	ALA4187445	ADME	Stability in mouse plasma assessed as parent compound remaining measured after 2 hrs post dose	Mus musculus	1	ALA4184209	cell-free format	Scientific Literature
20.	ALA4328866	ADME	Metabolic stability in mouse plasma assessed as parent compound remaining after 2 hrs	Mus musculus	2	ALA4325886	cell-free format	Scientific Literature