| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4178564 | B | Ratio of Kinact to Ki for recombinant human PAD2 expressed in Escherichia coli BL21(DE3)pLysS cells preincubated for 10 mins followed by BAEE substrate addition measured after 15 mins by COLDER assay | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
| 2. | ALA4178567 | B | Binding affinity to human PAD2 expressed in HEK293T cells assessed as protein occupancy preincubated for 15 mins followed by ionomycin addition measured after 1 hr in presence of CaCl2 by RIBFA probe based SDS-PAGE analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 3. | ALA4178568 | B | Binding affinity to human PAD2 expressed in HEK293T cells assessed as protein occupancy at 25 uM preincubated for 15 mins followed by ionomycin addition measured after 1 hr in presence of CaCl2 by RIBFA probe based SDS-PAGE analysis relative to control | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 4. | ALA4178569 | B | Binding affinity to human PAD2 expressed in HEK293T cells assessed as decrease in histone H3 citrulination incubated for 3 hrs in presence of ionomycin/CaCl2 by SDS-PAGE analysis | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 5. | ALA4178570 | A | Safety index, ratio of compound EC50 to BB-Cl-amide EC50 for cytotoxicity against HEK293T cells | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 6. | ALA4178575 | B | Selectivity ratio of Kinact/Ki for recombinant human PAD2 expressed in Escherichia coli BL21(DE3)pLysS cells to Kinact/Ki for recombinant full length N-terminal GST-tagged human PAD4 expressed in Escherichia coli Rosetta cells | Homo sapiens | 40 | cell-based format | Scientific Literature | ||
| 7. | ALA4178577 | B | Selectivity ratio of Kinact/Ki for recombinant His6-tagged PAD1 (unknown origin) to Kinact/Ki for recombinant His6-tagged PAD3 (unknown origin) | Homo sapiens | 19 | assay format | Scientific Literature | ||
| 8. | ALA4178578 | B | Selectivity ratio of Kinact/Ki for recombinant human PAD2 expressed in Escherichia coli BL21(DE3)pLysS to Kinact/Ki for recombinant His6-tagged PAD3 (unknown origin) | Homo sapiens | 23 | assay format | Scientific Literature | ||
| 9. | ALA4178579 | B | Selectivity ratio of Kinact/Ki for recombinant full length N-terminal GST-tagged human PAD4 expressed in Escherichia coli Rosetta to Kinact/Ki for recombinant His6-tagged PAD3 (unknown origin) | Homo sapiens | 19 | assay format | Scientific Literature | ||
| 10. | ALA4178580 | B | Selectivity ratio of Kinact/Ki for recombinant human PAD2 expressed in Escherichia coli BL21(DE3)pLysS cells to Kinact/Ki for recombinant His6-tagged PAD1 (unknown origin) | Homo sapiens | 4 | cell-based format | Scientific Literature |
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