| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4190757 | B | Inhibition of human DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells assessed as DNA ligation at 100 uM using fluorescently labelled ds nicked DNA as substrate after 30 mins by urea gel based imaging method relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 2. | ALA4190758 | B | Inhibition of human DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells assessed as reduction in fluorescently labelled ds nicked DNA substrate ligation at 100 uM after 30 mins by urea gel based imaging method relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 3. | ALA4190759 | B | Binding affinity to human full length N-terminal His-tagged DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells at 10 uM by fluorescence spectroscopic analysis | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 4. | ALA4190760 | F | Cytotoxicity against human HepG2 cells assessed as cell death at 10 uM after 48 hrs by MTT assay relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 5. | ALA4190761 | F | Cytotoxicity against human A549 cells assessed as cell death at 10 uM after 48 hrs by MTT assay relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 6. | ALA4190762 | F | Cytotoxicity against human HCT116 cells assessed as cell death at 10 uM after 48 hrs by MTT assay relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 7. | ALA4190763 | F | Cytotoxicity against human DLD1 cells assessed as cell death at 10 uM after 48 hrs by MTT assay relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 8. | ALA4190764 | F | Cytotoxicity against human MDA-MB-231 cells assessed as cell death at 10 uM after 48 hrs by MTT assay relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 9. | ALA4190765 | A | Cytotoxicity against HEK293 cells assessed as cell death at 10 uM after 48 hrs by MTT assay relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 10. | ALA4190766 | B | Inhibition of human DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells assessed as reduction in nick sealing activity using fluorescently labelled ds nicked DNA as substrate after 30 mins by urea gel based imaging method | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 11. | ALA4190767 | B | Binding affinity to human full length N-terminal His-tagged DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells at 1.25 to 5 uM by CD spectroscopic method | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 12. | ALA4190768 | B | Binding affinity to human N-terminal His-tagged DNA ligase 1 DBD expressed in Escherichia coli BL21(DE3)RP cells at 1.25 to 5 uM by CD spectroscopic method | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 13. | ALA4190769 | B | Binding affinity to human N-terminal His-tagged DNA ligase 1 DBD expressed in Escherichia coli BL21(DE3)RP cells at 10 uM by fluorescence spectroscopic analysis | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 14. | ALA4190770 | B | Binding affinity to human full length N-terminal His-tagged DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells assessed as destabilization of enzyme-nicked oligo DNA complex after 2 hrs by electrophoretic mobility shift assay | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 15. | ALA4190771 | B | Induction of linearized Escherichia coli pUC18 DNA intercalation up to 600 uM after 30 mins by agarose gel electrophoresis method | Escherichia coli | 1 | ALA4190183 | nucleic acid format | Scientific Literature | |
| 16. | ALA4190772 | B | Inhibition of EtBr binding to Escherichia coli pUC18 DNA up to 400 uM after 30 mins by fluorescence spectroscopic analysis | Escherichia coli | 1 | ALA4190183 | nucleic acid format | Scientific Literature | |
| 17. | ALA4190773 | F | Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 48 hrs by MTT assay | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 18. | ALA4190774 | F | Anticlonogenic activity against human HepG2 cells at >= 10 uM incubated for 48 hrs measured after 10 days by crystal violet staining based microscopy | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 19. | ALA4190775 | F | Induction of apoptosis in human HepG2 cells after 48 hrs by Annexin-V/PI staining based flow cytometry | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 20. | ALA4190776 | F | Cell cycle arrest in human HepG2 cells assessed as accumulation at G0/G1 phase by propidium iodide staining based flow cytometry | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature |
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