| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4190807 | B | Activation of ERbeta in mouse calvarial osteoblasts assessed as induction of osteoblast differentiation by measuring increase in ALP activity at 1 pM using PNPP as substrate after 48 hrs by colorimetric method | Mus musculus | 4 | single protein format | Scientific Literature | ||
| 2. | ALA4190808 | A | Cytotoxicity against mouse calvarial osteoblasts at 0.000001 to 1 uM after 48 hrs by MTT assay | Mus musculus | 4 | cell-based format | Scientific Literature | ||
| 3. | ALA4190809 | B | Activation of ERbeta in mouse calvarial osteoblasts assessed as induction of mineralization by measuring increase in calcium nodules at 1 pM supplemented with fresh medium containing compounds every 48 hrs for 18 days measured on day 18 by alizarin red-S staining based colorimetric method relative to control | Mus musculus | 1 | single protein format | Scientific Literature | ||
| 4. | ALA4190810 | F | Induction of osteoblast differentiation in mouse calvarial osteoblasts assessed as increase in BMP2 mRNA expression at 1 to 100 pM after 48 hrs by SYBR green1 staining based RT-qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4190811 | F | Induction of osteoblast differentiation in mouse calvarial osteoblasts assessed as increase in RUNX2 mRNA expression at 1 to 100 pM after 48 hrs by SYBR green1 staining based RT-qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4190812 | F | Induction of osteoblast differentiation in mouse calvarial osteoblasts assessed as increase in osterix mRNA expression at 1 to 100 pM after 48 hrs by SYBR green1 staining based RT-qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4190813 | B | Up regulation of ERalpha expression in mouse calvarial osteoblasts at 1 to 100 pM after 48 hrs by Western blot analysis | Mus musculus | 1 | assay format | Scientific Literature | ||
| 8. | ALA4190814 | B | Up regulation of ERbeta expression in mouse calvarial osteoblasts at 1 to 100 pM after 48 hrs by Western blot analysis | Mus musculus | 1 | assay format | Scientific Literature |
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