#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4189777	Binding	Displacement of fluorescein-labeled MLL4-43 from full length human menin expressed in Escherichia coli BL21 (DE3) cells after 3 hrs by fluorescence polarization assay	Homo sapiens	29	ALA4184328	cell-based format	Scientific Literature
2.	ALA4189778	Binding	Binding affinity to menin in mouse bone marrow cells harboring MLL-AF9 fusion protein assessed as inhibition of protein interaction with MLL by measuring cell growth inhibition after 7 days by MTT assay	Mus musculus	29	ALA4184328	cell-based format	Scientific Literature
3.	ALA4189779	ADME	Half life in CD-1 mouse liver microsomes at 1 uM after 3 to 60 mins by LC-MS/MS method	Mus musculus	29	ALA4184328	microsome format	Scientific Literature
4.	ALA4189780	Unassigned	Selectivity index, ratio of GI50 for mouse bone marrow cells transformed with control oncogenes Hoxa9/Meis1 to GI50 for binding affinity to menin in mouse bone marrow cells harboring MLL-AF9 fusion protein assessed as inhibition of protein interaction with MLL by measuring cell growth inhibition	Mus musculus	3	ALA4184328	cell-based format	Scientific Literature
5.	ALA4189781	Binding	Binding affinity to human C-terminal Avi-tagged menin expressed in Escherichia coli BL21 (DE3) cells by bio-layer interferometry	Homo sapiens	2	ALA4184328	cell-based format	Scientific Literature
6.	ALA4189782	ADME	Cytotoxicity against mouse bone marrow cells transformed with control oncogenes Hoxa9/Meis1 assessed as cell growth inhibition after 7 days by MTT assay	Mus musculus	1	ALA4184328	cell-based format	Scientific Literature
7.	ALA4189783	Functional	Cytotoxicity against human MV411 cells harboring MLL-AF4 fusion protein assessed as cell growth inhibition after 7 days by MTT assay	Homo sapiens	2	ALA4184328	cell-based format	Scientific Literature
8.	ALA4189784	Functional	Cytotoxicity against human MOLM13 cells harboring MLL-AF9 fusion protein assessed as cell growth inhibition after 7 days by MTT assay	Homo sapiens	2	ALA4184328	cell-based format	Scientific Literature
9.	ALA4189785	ADME	Cytotoxicity against human K562 cells assessed as cell growth inhibition after 7 days by MTT assay	Homo sapiens	1	ALA4184328	cell-based format	Scientific Literature
10.	ALA4189786	ADME	Cytotoxicity against human U937 cells assessed as cell growth inhibition after 7 days by MTT assay	Homo sapiens	1	ALA4184328	cell-based format	Scientific Literature
11.	ALA4189787	Binding	Binding affinity to menin in human MV411 cells assessed as inhibition of protein interaction with AF4-fused MLL by measuring downregulation of HOXA9 gene expression at 100 nM after 6 days by qRT-PCR method	Homo sapiens	1	ALA4184328	cell-based format	Scientific Literature
12.	ALA4189788	Binding	Binding affinity to menin in human MV411 cells assessed as inhibition of protein interaction with AF4-fused MLL by measuring downregulation of MEIS1 gene expression at 100 nM after 6 days by qRT-PCR method	Homo sapiens	1	ALA4184328	cell-based format	Scientific Literature
13.	ALA4189789	Binding	Binding affinity to menin in human MV411 cells assessed as inhibition of protein interaction with AF4-fused MLL by measuring downregulation of DLX2 gene expression at 100 nM after 6 days by qRT-PCR method	Homo sapiens	1	ALA4184328	cell-based format	Scientific Literature
14.	ALA4189790	Binding	Binding affinity to menin in human MV411 cells assessed as inhibition of protein interaction with AF4-fused MLL by measuring upregulation of ITGAM gene expression at 50 to 200 nM after 6 days by qRT-PCR method	Homo sapiens	1	ALA4184328	cell-based format	Scientific Literature
15.	ALA4189791	ADME	Oral bioavailability in C57BL/6 mouse at 30 mg/kg by LC-MS/MS method	Mus musculus	1	ALA4184328	organism-based format	Scientific Literature
16.	ALA4189792	Toxicity	Toxicity in NSG mouse xenografted with luciferase expressing human MV411 cells assessed as reduction in body weight at 80 mg/kg, ip administered for 21 consecutive days measured during compound dosing	Mus musculus	1	ALA4184328	organism-based format	Scientific Literature
17.	ALA4189793	Functional	In vivo inhibition of menin interaction with MLL in luciferase expressing human MV411 cells xenografted in NSG mouse at 80 mg/kg, ip administered for 21 consecutive days measured at days 7, 14, 21 during compound dosing by bioluminescence method relative to vehicle-treated control	Mus musculus	1	ALA4184328	organism-based format	Scientific Literature
18.	ALA4189794	Functional	Antileukemia activity against luciferase expressing human MV411 cells xenografted in NSG mouse assessed as reduction in spleen weight at 80 mg/kg, ip administered for 6 consecutive days	Homo sapiens	1	ALA4184328	organism-based format	Scientific Literature
19.	ALA4189795	Functional	Antileukemia activity against luciferase expressing human MV411 cells xenografted in NSG mouse assessed as reduction in spleen size at 80 mg/kg, ip administered for 6 consecutive days	Homo sapiens	1	ALA4184328	organism-based format	Scientific Literature
20.	ALA4189796	Functional	Antileukemia activity against luciferase expressing human MV411 cells xenografted in NSG mouse assessed as levels of human CD45positive cells at 80 mg/kg, ip administered for 6 consecutive days by flow cytometric method (Rvb = 75%)	Homo sapiens	1	ALA4184328	organism-based format	Scientific Literature