| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4186205 | B | Inhibition of recombinant Salmonella typhimurium N-terminal His6-SUMO-tagged DHp-catalytic domain PhoQ (257 to 487 residues) autophosphorylation expressed in Escherichia coli BL21 (DE3) preincubated for 10 mins followed by [gamma-32P]-ATP addition after 30 mins by phosphotransferase dot blot assay | Salmonella enterica subsp. enterica serovar Typhimurium | 13 | assay format | Scientific Literature | ||
| 2. | ALA4186208 | B | Inhibition of recombinant Salmonella typhimurium N-terminal His6-SUMO-tagged DHp-catalytic domain PhoQ (257 to 487 residues) autophosphorylation expressed in Escherichia coli BL21(DE3) at 500 uM preincubated for 10 mins followed by [gamma-32P]-ATP addition after 30 mins by phosphotransferase dot blot assay relative to control | Salmonella enterica subsp. enterica serovar Typhimurium | 15 | assay format | Scientific Literature | ||
| 3. | ALA4186209 | B | Inhibition of recombinant Caulobacter vibrioides N-terminal His6-tagged DHp-catalytic domain DivJ (188 to 585 residues) autophosphorylation expressed in Escherichia coli BL21(DE3) at 500 uM preincubated for 10 mins followed by [gamma-32P]-ATP addition after 15 mins by phosphotransferase dot blot assay relative to control | Caulobacter vibrioides | 15 | assay format | Scientific Literature | ||
| 4. | ALA4186216 | A | Hemolytic activity against sheep RBC at 0.5 mM after 30 mins relative to control | Ovis aries | 15 | cell-based format | Scientific Literature | ||
| 5. | ALA4186219 | F | Antibacterial activity against Escherichia coli DC2 after 24 hrs by broth dilution method | Escherichia coli | 15 | organism-based format | Scientific Literature | ||
| 6. | ALA4186220 | F | Antibacterial activity against Bacillus subtilis YB886 after 24 hrs by broth dilution method | Bacillus subtilis | 15 | organism-based format | Scientific Literature | ||
| 7. | ALA4186221 | F | Antibacterial activity against Caulobacter crescentus NA1000 after 24 hrs by broth dilution method | Caulobacter vibrioides | 15 | organism-based format | Scientific Literature | ||
| 8. | ALA4186231 | B | Binding affinity to recombinant Caulobacter vibrioides cell cycle histidine kinase CckA delta 3 mutant DHp domain (190 to 562 residues) expressed in Escherichia coli assessed as change in metlting temperature at 30 uM after 2 mins by SYPRO orange dye based DSF assay | Caulobacter vibrioides | 16 | assay format | Scientific Literature | ||
| 9. | ALA4186232 | B | Inhibition of recombinant Caulobacter vibrioides cell cycle histidine kinase CckA deltaTM mutant DHp domain (70 to 691 residues) expressed in Escherichia coli in presence of varying levels of ATP measured every 60 secs for 2 hrs by PK/LDH enzyme coupled assay | Caulobacter vibrioides | 16 | assay format | Scientific Literature | ||
| 10. | ALA4186235 | B | Inhibition of recombinant Caulobacter vibrioides N-terminal His6-tagged DHp-catalytic domain DivJ (188 to 585 residues) expressed in Escherichia coli BL21 (DE3) preincubated for 10 mins followed by [gamma-32P]-ATP addition after 15 mins by phosphotransferase dot blot assay | Caulobacter vibrioides | 10 | assay format | Scientific Literature |
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