# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4190487 | B | Proteolysis targeting chimera activity in human MCF7 cells assessed as induction of VHL-mediated delivery of ERRalpha for protein degradation by proteasome by measuring drug level causing 50% substrate depletion incubated for 24 hrs by Western blot method | Homo sapiens | 1 | ALA4190166 | cell-based format | Scientific Literature | |
2. | ALA4190488 | F | In vivo induction of ERRalpha degradation in CD-1 mouse heart at 100 mg/kg, ip every 8 hrs measured 5 hrs post last dose by LC/MS analysis relative to control | Mus musculus | 1 | ALA4190166 | organism-based format | Scientific Literature | |
3. | ALA4190489 | F | In vivo induction of ERRalpha degradation in CD-1 mouse kidney at 100 mg/kg, ip every 8 hrs measured 5 hrs post last dose by LC/MS analysis relative to control | Mus musculus | 1 | ALA4190166 | organism-based format | Scientific Literature | |
4. | ALA4190490 | F | In vivo induction of ERRalpha degradation in human MDA-MB-231 cells xenografted in CD-1 mouse at 100 mg/kg, ip every 8 hrs measured 5 hrs post last dose by LC/MS analysis relative to control | Homo sapiens | 1 | ALA4190166 | organism-based format | Scientific Literature | |
5. | ALA4305574 | B | Binding affinity to GST-tagged ERRalpha-LBD (unknown origin) using fluorescein-conjugated coactivator PGC-1alpha incubated for 1 hr by TR-FRET assay | Homo sapiens | 20 | ALA4304753 | single protein format | Scientific Literature | |
6. | ALA4305576 | B | Protac activity at VHL/ERRalpha in human MDA-MB-231 cells assessed as induction of ERalpha degradation at 30 nM incubated for 4 hrs by Western blot analysis relative to control | Homo sapiens | 12 | ALA4304753 | cell-based format | Scientific Literature | |
7. | ALA4305578 | B | Protac activity at VHL/ERRalpha in human MDA-MB-231 cells assessed as induction of ERalpha degradation at 100 nM incubated for 4 hrs by Western blot analysis relative to control | Homo sapiens | 12 | ALA4304753 | cell-based format | Scientific Literature | |
8. | ALA4388596 | B | Protac activity at VHL/ERRalpha in epoxomicin pre-treated human MCF7 cells assessed as induction of ERRalpha protein degradation incubated for 8 hrs by Western blot analysis | Homo sapiens | 1 | ALA4387721 | cell-based format | Scientific Literature | |
9. | ALA4388597 | B | Protac activity at VHL/ERRalpha in epoxomicin pre-treated human MCF7 cells assessed as induction of ERRalpha protein degradation at 1 uM incubated for 8 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA4387721 | cell-based format | Scientific Literature | |
10. | ALA5246301 | B | Protac activity at VHL/ERRalpha in human MCF7 cells | Homo sapiens | 1 | ALA5244258 | cell-based format | Scientific Literature | |
11. | ALA5246302 | B | Protac activity at VHL/ERRalpha in human MCF7 cells assessed as maximum efficacy relative to control | Homo sapiens | 1 | ALA5244258 | cell-based format | Scientific Literature | |
12. | ALA5246312 | B | Protac activity at ER/VHL (unknown origin) degradation in human MCF7 cells by immunoblotting analysis | Homo sapiens | 1 | ALA5244258 | cell-based format | Scientific Literature | |
13. | ALA5246313 | B | Protac activity at ER/VHL (unknown origin) degradation in human MCF7 cells assessed as maximum efficacy by immunoblotting analysis relative to control | Homo sapiens | 1 | ALA5244258 | cell-based format | Scientific Literature |