# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4219513 | B | Proteolysis targeting chimera activity in human HeLa cells assessed as induction of VCB-mediated delivery of BRD4 short isoform for protein degradation by proteasome by measuring drug level causing 50% cellular protein depletion incubated for 24 hrs by Western blotting method | Homo sapiens | 7 | ALA4219029 | cell-based format | Scientific Literature | |
2. | ALA4219514 | B | Proteolysis targeting chimera activity in human HeLa cells assessed as induction of VCB-mediated delivery of BRD4 long isoform for protein degradation by proteasome by measuring drug level causing 50% cellular protein depletion incubated for 24 hrs by Western blotting method | Homo sapiens | 7 | ALA4219029 | cell-based format | Scientific Literature | |
3. | ALA4219515 | B | Proteolysis targeting chimera activity in human HeLa cells assessed as induction of VCB-mediated delivery of BRD3 for protein degradation by proteasome by measuring drug level causing 50% cellular protein depletion incubated for 24 hrs by Western blotting method | Homo sapiens | 7 | ALA4219029 | cell-based format | Scientific Literature | |
4. | ALA4219516 | B | Proteolysis targeting chimera activity in human HeLa cells assessed as induction of VCB-mediated delivery of BRD2 for protein degradation by proteasome by measuring drug level causing 50% cellular protein depletion incubated for 24 hrs by Western blotting method | Homo sapiens | 5 | ALA4219029 | cell-based format | Scientific Literature | |
5. | ALA4219517 | B | Proteolysis targeting chimera activity in human MV4-11 cells assessed as induction of VCB-mediated delivery of BRD4 for protein degradation by proteasome by measuring cellular protein depletion at 50 nM incubated for 4 hrs by Western blotting method | Homo sapiens | 9 | ALA4219029 | cell-based format | Scientific Literature | |
6. | ALA4219518 | F | Antiproliferative activity against human HL60 cells after 48 hrs by CellTiter-Glo luminescent cell viability assay | Homo sapiens | 9 | ALA4219029 | cell-based format | Scientific Literature | |
7. | ALA4219519 | B | Proteolysis targeting chimera activity in human HeLa cells assessed as induction of VCB-mediated delivery of BRD4 short isoform for protein degradation by proteasome by measuring drug level causing 50% cellular protein depletion incubated for 24 hrs by Western blotting method relative to control | Homo sapiens | 7 | ALA4219029 | cell-based format | Scientific Literature | |
8. | ALA4219520 | B | Proteolysis targeting chimera activity in human HeLa cells assessed as induction of VCB-mediated delivery of BRD4 long isoform for protein degradation by proteasome by measuring drug level causing 50% cellular protein depletion incubated for 24 hrs by Western blotting method relative to control | Homo sapiens | 7 | ALA4219029 | cell-based format | Scientific Literature | |
9. | ALA4219521 | B | Proteolysis targeting chimera activity in human HeLa cells assessed as induction of VCB-mediated delivery of BRD3 for protein degradation by proteasome by measuring drug level causing 50% cellular protein depletion incubated for 24 hrs by Western blotting method relative to control | Homo sapiens | 7 | ALA4219029 | cell-based format | Scientific Literature | |
10. | ALA4219522 | B | Proteolysis targeting chimera activity in human HeLa cells assessed as induction of VCB-mediated delivery of BRD2 for protein degradation by proteasome by measuring drug level causing 50% cellular protein depletion incubated for 24 hrs by Western blotting method relative to control | Homo sapiens | 7 | ALA4219029 | cell-based format | Scientific Literature | |
11. | ALA4219523 | F | Antiproliferative activity against human MV4-11 cells after 48 hrs by CellTiter-Glo luminescent cell viability assay | Homo sapiens | 9 | ALA4219029 | cell-based format | Scientific Literature | |
12. | ALA4219524 | B | Proteolysis targeting chimera activity in human MV4-11 cells assessed as induction of VCB-mediated delivery of BRD4 for protein degradation by proteasome by measuring cellular cMyc protein depletion at 50 nM incubated for 4 hrs by Western blotting method | Homo sapiens | 9 | ALA4219029 | cell-based format | Scientific Literature | |
13. | ALA4219525 | B | Proteolysis targeting chimera activity in human HL60 cells assessed as induction of VCB-mediated delivery of BRD4 for protein degradation by proteasome by measuring cellular protein depletion at 50 nM incubated for 4 hrs by Western blotting method | Homo sapiens | 9 | ALA4219029 | cell-based format | Scientific Literature | |
14. | ALA4219526 | B | Proteolysis targeting chimera activity in human HL60 cells assessed as induction of VCB-mediated delivery of BRD4 for protein degradation by proteasome by measuring cellular cMyc protein depletion at 50 nM incubated for 4 hrs by Western blotting method | Homo sapiens | 9 | ALA4219029 | cell-based format | Scientific Literature | |
15. | ALA4219527 | B | Binding affinity to N-terminal His6-tagged recombinant human Brd4 BD2 expressed in Escherichia coli BL21 (DE3) assessed as compound binary complex formation by isothermal titration calorimetry-based assay | Homo sapiens | 6 | ALA4219029 | assay format | Scientific Literature | |
16. | ALA4219528 | B | Binding affinity to N-terminal His6-tagged recombinant human VHL (54 to 213 residues)/elongin C (17 to 112 residues)/elongin B (1 to 104 residues) expressed in Escherichia coli BL21 (DE3) assessed as compound binary complex formation by isothermal titration calorimetry-based assay | Homo sapiens | 6 | ALA4219029 | assay format | Scientific Literature | |
17. | ALA4219529 | B | Induction of interaction of N-terminal His6-tagged recombinant human VHL (54 to 213 residues)/elongin C (17 to 112 residues)/elongin B (1 to 104 residues) co-expressed in Escherichia coli BL21 (DE3) with recombinant human Brd4 BD2 assessed as VCB/BRD4/compound ternary complex formation by isothermal titration calorimetry-based assay | Homo sapiens | 6 | ALA4219029 | assay format | Scientific Literature |