# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4221678 | B | Uncompetitive inhibition of recombinant human cathepsin B endopeptidase activity assessed as inhibitory constant for enzyme-substrate-inhibitor complex using Z-Arg-Arg-AMC as substrate in presence of pH 6 phosphate buffer by fluorescence assay | Homo sapiens | 9 | ALA4219118 | single protein format | Scientific Literature | |
2. | ALA4221679 | B | Mixed inhibition of recombinant human cathepsin B exopeptidase activity assessed as inhibitory constant for enzyme-inhibitor complex using Abz-Gly-Ile-Val-Arg-Ala-Lys(Dnp)-OH as substrate in presence of pH 5 acetate buffer by fluorescence assay | Homo sapiens | 8 | ALA4219118 | single protein format | Scientific Literature | |
3. | ALA4221680 | B | Mixed inhibition of recombinant human cathepsin B exopeptidase activity assessed as inhibitory constant for enzyme-substrate-inhibitor complex using Abz-Gly-Ile-Val-Arg-Ala-Lys(Dnp)-OH as substrate in presence of pH 5 acetate buffer by fluorescence assay | Homo sapiens | 8 | ALA4219118 | single protein format | Scientific Literature | |
4. | ALA4221686 | B | Competitive inhibition of recombinant human cathepsin B endopeptidase activity assessed as inhibitory constant for enzyme-inhibitor complex using Z-Arg-Arg-AMC as substrate in presence of pH 4.5 acetate buffer by fluorescence assay | Homo sapiens | 10 | ALA4219118 | single protein format | Scientific Literature | |
5. | ALA4221687 | B | Inhibition of cathepsin B in human MCF-10A neoT cells assessed as proteolysis of extracellular membrane by measuring decrease in extracellular degradation of DQ-collagen 4 at 5 uM incubated for 6 hrs measured for 2 hrs by fluorescence based spectrofluorimetric assay relative to control | Homo sapiens | 4 | ALA4219118 | cell-based format | Scientific Literature | |
6. | ALA4221690 | B | Inhibition of cathepsin B in human MCF-10A neoT cells assessed as proteolysis of extracellular membrane by measuring decrease in intracellular degradation of DQ-collagen 4 at 50 uM incubated for 2 hrs followed by DQ-collagen addition measured for 2 hrs by propidium iodide staining based flow cytometry relative to control | Homo sapiens | 6 | ALA4219118 | cell-based format | Scientific Literature | |
7. | ALA4221693 | B | Uncompetitive inhibition of human liver cathepsin H assessed as inhibitory constant for enzyme-substrate-inhibitor complex using R-AMC as substrate in presence of pH 6 phosphate buffer by fluorescence assay | Homo sapiens | 1 | ALA4219118 | assay format | Scientific Literature | |
8. | ALA4221695 | B | Non-competitive inhibition of recombinant human cathepsin L expressed in Escherichia coli assessed as inhibitory constant for enzyme-inhibitor complex using Z-FR-AMC as substrate in presence of pH 6 phosphate buffer by fluorescence assay | Homo sapiens | 1 | ALA4219118 | assay format | Scientific Literature | |
9. | ALA4221698 | B | Non-competitive inhibition of recombinant human cathepsin L expressed in Escherichia coli assessed as inhibitory constant for enzyme-substrate-inhibitor complex using Z-FR-AMC as substrate in presence of pH 6 phosphate buffer by fluorescence assay | Homo sapiens | 1 | ALA4219118 | assay format | Scientific Literature | |
10. | ALA4221700 | F | Antiinvasive activity against human MCF10AneoT cells at 5 uM incubated for 1 hr measured every 15 mins for 72 hrs by real time xCELLigence assay relative to control | Homo sapiens | 3 | ALA4219118 | cell-based format | Scientific Literature | |
11. | ALA4221711 | A | Cytotoxicity against human MCF10AneoT cells assessed as decrease in cell viability after 72 hrs by MTS assay | Homo sapiens | 7 | ALA4219118 | cell-based format | Scientific Literature | |
12. | ALA4221712 | A | Cytotoxicity against human U87MG cells assessed as decrease in cell viability after 72 hrs by MTS assay | Homo sapiens | 7 | ALA4219118 | cell-based format | Scientific Literature |