# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4221466 | B | Inhibition of sclerostin binding to LRP6 (unknown origin) expressed in rat MC3T3-E1 cells co-expressing TCF/LEF luciferase plasmid assessed as reduction in sclerostin-induced inhibition of Wnt3a activation by measuring luciferase activity at 3 uM after 24 hrs by dual-luciferase reporter gene assay (Rvb = 100 +/- 3.1%) | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
2. | ALA4221467 | B | Inhibition of sclerostin binding to human ALP-fused LRP5 at 50 uM using alkaline phosphatase as substrate pretreated for 2 hrs followed by substrate addition measured after 25 mins by ELISA relative to control | Homo sapiens | 5 | single protein format | Scientific Literature | ||
3. | ALA4221468 | B | Inhibition of sclerostin binding to LRP6 (unknown origin) expressed in rat MC3T3-E1 cells co-expressing TCF/LEF luciferase plasmid assessed as reduction in sclerostin-induced inhibition of Wnt3a activation by measuring luciferase activity at 0.3 uM after 24 hrs by dual-luciferase reporter gene assay (Rvb = 100 +/- 2.6%) | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
4. | ALA4221469 | B | Inhibition of sclerostin binding to LRP6 (unknown origin) expressed in rat MC3T3-E1 cells co-expressing TCF/LEF luciferase plasmid assessed as reduction in sclerostin-induced inhibition of Wnt3a activation by measuring increase in luciferase activity after 24 hrs by dual-luciferase reporter gene assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
5. | ALA4221470 | B | Inhibition of sclerostin binding to LRP5/6 in mouse ST-2 cells assessed as reduction in sclerostin-induced inhibition of Wnt-mediated signaling activity at 3 to 10 uM after 3 days by alkaline phosphatase dye based microscopic analysis | Mus musculus | 1 | cell-based format | Scientific Literature |