# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4221643 | B | Activation of rabbit skeletal muscle PP1 catalytic subunit at 19 to 395 uM using [32P]MLC20 as substrate pretreated for 10 mins followed by substrate addition measured after 10 mins by scintillation counting method relative to control | Oryctolagus cuniculus | 16 | ALA4219114 | assay format | Scientific Literature | |
2. | ALA4221644 | B | Activation of rabbit skeletal muscle PP2A catalytic subunit at 19 to 395 uM using [32P]MLC20 as substrate pretreated for 10 mins followed by substrate addition measured after 10 mins by scintillation counting method relative to control | Oryctolagus cuniculus | 16 | ALA4219114 | assay format | Scientific Literature | |
3. | ALA4221647 | B | Activation of rabbit skeletal muscle PP1 catalytic subunit using [32P]MLC20 as substrate pretreated for 10 mins followed by substrate addition measured after 10 mins by scintillation counting method relative to control | Oryctolagus cuniculus | 5 | ALA4219114 | assay format | Scientific Literature | |
4. | ALA4221648 | B | Activation of rabbit skeletal muscle PP2A catalytic subunit using [32P]MLC20 as substrate pretreated for 10 mins followed by substrate addition measured after 10 mins by scintillation counting method relative to control | Oryctolagus cuniculus | 5 | ALA4219114 | assay format | Scientific Literature | |
5. | ALA4221649 | B | Activation of rabbit skeletal muscle PP1 catalytic subunit at 395 uM using [32P]MLC20 as substrate pretreated for 10 mins followed by substrate addition measured after 10 mins in presence of 10 to 25 uM MYPT1 (23 to 38 residues) peptide by scintillation counting method | Oryctolagus cuniculus | 2 | ALA4219114 | assay format | Scientific Literature | |
6. | ALA4221650 | B | Activation of rabbit skeletal muscle PP2A catalytic subunit at 395 uM using [32P]MLC20 as substrate pretreated for 10 mins followed by substrate addition measured after 10 mins in presence of 10 uM MYPT1 (23 to 38 residues) peptide by scintillation counting method relative to control | Oryctolagus cuniculus | 2 | ALA4219114 | assay format | Scientific Literature | |
7. | ALA4221651 | B | Binding affinity to full length recombinant human N-terminal His6-tagged PP1cdelta expressed in Escherichia coli M15 assessed as inhibition of protein interaction with GST-tagged MYPT1 at 10 to 50 uM by surface plasmon resonance method | Homo sapiens | 2 | ALA4219114 | assay format | Scientific Literature | |
8. | ALA4221653 | B | Activation of PP1 catalytic subunit/PP2A catalytic subunit in human HeLa cells at >100 uM using [32P]MLC20 as substrate pretreated for 1 hr followed by substrate addition measured after 30 secs by scintillation counting method | Homo sapiens | 1 | ALA4219114 | cell-based format | Scientific Literature | |
9. | ALA4221655 | F | Cytotoxicity against human HeLa cells assessed as reduction in cell viability at >200 uM after 24 hrs by Alamar blue assay | Homo sapiens | 4 | ALA4219114 | cell-based format | Scientific Literature |