| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4235267 | B | Antagonist activity at recombinant human ERalpha expressed in HEK293 cells assessed as inhibition of estradiol-induced YFP-fused SRC1 coactivator recruitment measured after 1 hr in presence of Coelenterazine H by BRET assay | Homo sapiens | 5 | ALA4229504 | cell-based format | Scientific Literature | |
| 2. | ALA4235268 | A | Partial agonist activity at recombinant human ERalpha expressed in HEK293 cells assessed as YFP-fused SRC1 coactivator recruitment measured after 1 hr in presence of Coelenterazine H by BRET assay | Homo sapiens | 5 | ALA4229504 | cell-based format | Scientific Literature | |
| 3. | ALA4235269 | B | Inhibition of full length recombinant human N-terminal GST-tagged HDAC6 expressed in Sf9 cells using Ac-Leu-Gly-Lys(Ac)-AMC as substrate measured after 30 mins by fluorescence assay | Homo sapiens | 6 | ALA4229504 | cell-based format | Scientific Literature | |
| 4. | ALA4235270 | B | Inhibition of human full length C-terminal His-tagged HDAC3/N-terminal GST-tagged human NCOR2 (395 to 489 residues) expressed in baculovirus expression system using Ac-Leu-Gly-Lys(Ac)-AMC as substrate measured after 30 mins by fluorescence assay | Homo sapiens | 5 | ALA4229504 | assay format | Scientific Literature | |
| 5. | ALA4235271 | F | Cytotoxicity against ER positive human MCF7 cells treated every 2 days for 4 days measured post last dose by CellTiter-Glo luminescence assay | Homo sapiens | 6 | ALA4229504 | cell-based format | Scientific Literature | |
| 6. | ALA4235272 | F | Cytotoxicity against ER positive human MCF7 cells treated every 2 days for 4 days measured after 7 days by CellTiter-Glo luminescence assay | Homo sapiens | 6 | ALA4229504 | cell-based format | Scientific Literature | |
| 7. | ALA4235273 | F | Cytotoxicity against ER negative human MDA-MB-231 cells treated every 2 days for 4 days measured post last dose by CellTiter-Glo luminescence assay | Homo sapiens | 3 | ALA4229504 | cell-based format | Scientific Literature | |
| 8. | ALA4235274 | F | Cytotoxicity against ER negative human MDA-MB-231 cells treated every 2 days for 4 days measured after 7 days by CellTiter-Glo luminescence assay | Homo sapiens | 3 | ALA4229504 | cell-based format | Scientific Literature | |
| 9. | ALA4235275 | F | Cytotoxicity against ER negative human MCF10A cells treated every 2 days for 4 days measured post last dose by CellTiter-Glo luminescence assay | Homo sapiens | 3 | ALA4229504 | cell-based format | Scientific Literature | |
| 10. | ALA4235276 | F | Cytotoxicity against ER negative human MCF10A cells treated every 2 days for 4 days measured after 7 days by CellTiter-Glo luminescence assay | Homo sapiens | 3 | ALA4229504 | cell-based format | Scientific Literature | |
| 11. | ALA4235277 | B | Inhibition of HDAC3 in human MCF7 cells assessed as increase in acetylated histone H4 expression after 8 hrs by Western blot analysis | Homo sapiens | 2 | ALA4229504 | cell-based format | Scientific Literature | |
| 12. | ALA4235278 | B | Inhibition of HDAC6 in human MCF7 cells assessed as increase in acetylated alpha-tubulin expression after 8 hrs by Western blot analysis | Homo sapiens | 2 | ALA4229504 | cell-based format | Scientific Literature | |
| 13. | ALA4235281 | B | Antagonist activity at ERalpha in human MCF7 cells assessed as down regulation of ESR1 expression at 0.1 to 9 uM after 8 hrs by Western blot analysis | Homo sapiens | 2 | ALA4229504 | cell-based format | Scientific Literature | |
| 14. | ALA4235282 | B | Antagonist activity at ERalpha in human MCF7 cells assessed as suppression of MYC mRNA expression at 5 uM after 24 hrs by QIAzol reagent based RT-qPCR analysis | Homo sapiens | 3 | ALA4229504 | cell-based format | Scientific Literature | |
| 15. | ALA4235283 | B | Antagonist activity at ESR1 in human MCF7 cells assessed as suppression of ESR1 mRNA expression at 5 uM after 24 hrs by QIAzol reagent based RT-qPCR analysis | Homo sapiens | 3 | ALA4229504 | cell-based format | Scientific Literature | |
| 16. | ALA4235284 | B | Antagonist activity at ERalpha in human MCF7 cells assessed as suppression of GREB1 mRNA expression at 5 uM after 24 hrs by QIAzol reagent based RT-qPCR analysis | Homo sapiens | 3 | ALA4229504 | cell-based format | Scientific Literature | |
| 17. | ALA4235285 | B | Antagonist activity at ERalpha in human MCF7 cells assessed as suppression of TFF1 mRNA expression at 5 uM after 24 hrs by QIAzol reagent based RT-qPCR analysis | Homo sapiens | 3 | ALA4229504 | cell-based format | Scientific Literature | |
| 18. | ALA4235286 | B | Inhibition of HDAC6/HDAC3 in human MCF7 cells assessed as induction of SREBF1 mRNA expression at 5 uM after 24 hrs by QIAzol reagent based RT-qPCR analysis | Homo sapiens | 2 | ALA4229504 | cell-based format | Scientific Literature | |
| 19. | ALA4235287 | B | Inhibition of HDAC6/HDAC3 in human MCF7 cells assessed as induction of CTGF mRNA expression at 5 uM after 24 hrs by QIAzol reagent based RT-qPCR analysis | Homo sapiens | 2 | ALA4229504 | cell-based format | Scientific Literature | |
| 20. | ALA4235288 | B | Inhibition of HDAC6/HDAC3 in human MCF7 cells assessed as induction of CDKN1A mRNA expression at 5 uM after 24 hrs by QIAzol reagent based RT-qPCR analysis | Homo sapiens | 2 | ALA4229504 | cell-based format | Scientific Literature |
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