# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA664657 | F | In vivo hypotension in anesthetized dog expressed as the dose of the drug producing a 25% decrease in DAP from control valueNo effect at a maximal concentration of 50 uM | Canis lupus familiaris | 1 | organism-based format | Scientific Literature | ||
2. | ALA672598 | F | In vivo contractility in anesthetized dog expressed as dose which produced a 50% increase in dP/dt max from the control value. | Canis lupus familiaris | 37 | organism-based format | Scientific Literature | ||
3. | ALA668025 | F | The compound was tested in vivo for hypotension in anesthetized dog expressed as % decrease in diastolic arterial pressure (DAP); No effect at a maximum intravenous dose. | Canis lupus familiaris | 8 | organism-based format | Scientific Literature | ||
4. | ALA670334 | F | The compound was tested in vivo for contractility in anesthetized dog expressed as % dP/dt max;No effect at a maximum intravenous dose. | Canis lupus familiaris | 2 | organism-based format | Scientific Literature | ||
5. | ALA688554 | F | In vitro inotropic activity in isolated left atria from guinea pig. | Cavia porcellus | 29 | organism-based format | Scientific Literature | ||
6. | ALA688722 | F | In vitro inotropic activity expressed as the maximal increase in developed tension in isolated left atria from guinea pig. | Cavia porcellus | 31 | organism-based format | Scientific Literature | ||
7. | ALA690329 | F | The compound was tested in vitro for chronotropic activity in isolated right atria from guinea pig;No effect at a maximal concentration of 50 uM | Cavia porcellus | 2 | organism-based format | Scientific Literature | ||
8. | ALA688619 | F | The compound was tested in vitro for chronotropic activity expressed as the maximal increase in beat/min in isolated left atria from guinea pig;No effect at a maximal concentration of 50 uM | Cavia porcellus | 2 | organism-based format | Scientific Literature | ||
9. | ALA3705193 | B | FRET Assay: We have developed effective assays to monitor the inhibition of CBFβ-SMMHC binding to the Runt domain (as well as for CBFβ binding to the Runt domain). We fused the green fluorescent protein derivative Cerulean to the N-terminus of the Runt domain and the green fluorescent protein derivative Venus to the N-terminus of CBFβ-SMMHC (as well as to CBFβ (Gorczynski, Grembecka et al. 2007)). The ratio of the emission intensities at 525 nm and 474 nm, measured after excitation at 433 nm, was used as the readout in this assay. The dynamic range for the FRET assay was determined by adding a 30-fold excess of untagged CBFβ-SMMHC (or CBFβ in the case of CBFβ Runt domain binding) to the assay and the associated change in the FRET ratio was defined as 100% inhibition. | Homo sapiens | 34 | single protein format | BindingDB Database |