# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4232621 | B | Inhibition of SAH-rhodamine binding to NNMT in human 786-O cell soluble proteomic lysate at 2 uM preincubated for 1 hr followed by SAH-rhodamine addition measured after 5 mins by Gel-based ABPP analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
2. | ALA4232623 | B | Inhibition of SAH-rhodamine binding to NNMT in human 786-O cell soluble proteomic lysate at 0.5 to 2 uM preincubated for 1 hr followed by SAH-rhodamine addition measured after 5 mins by Gel-based ABPP analysis | Homo sapiens | 38 | assay format | Scientific Literature | ||
3. | ALA4232624 | B | Inhibition of SAH-rhodamine binding to NNMT in human 786-O cell soluble proteomic lysate preincubated for 1 hr followed by SAH-rhodamine addition measured after 5 mins by Gel-based ABPP analysis | Homo sapiens | 11 | assay format | Scientific Literature | ||
4. | ALA4232625 | B | Inhibition of NNMT in human 786-O cell lysate using d4-nicotinamide as substrate preincubated for 1 hr followed by substrate and SAM addition measured after 15 to 30 mins by LC-MS analysis | Homo sapiens | 3 | assay format | Scientific Literature | ||
5. | ALA4232626 | B | Inhibition of NNMT C165A mutant in human 786-O cell lysate using d4-nicotinamide as substrate preincubated for 1 hr followed by substrate and SAM addition measured after 15 to 30 mins by LC-MS analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
6. | ALA4232627 | B | Inhibition of SAH-rhodamine binding to NNMT in human 786-O cell soluble proteomic lysate assessed as ratio of observed rate constant to test compound concentration preincubated for 10 to 40 mins followed by SAH-rhodamine addition measured after 5 mins by Gel-based ABPP analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
7. | ALA4232633 | B | Binding affinity to NNMT C165 residue in human 786-O cell lysate at 5 uM incubated for 4 hrs followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
8. | ALA4232634 | B | Binding affinity to NNMT C165 residue in human 786-O cell soluble proteomic lysate at 1 to 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
9. | ALA4232635 | B | Binding affinity to NNMT C165 residue in human 786-O cell soluble proteomic lysate at 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis relative to control | Homo sapiens | 2 | assay format | Scientific Literature | ||
10. | ALA4232636 | B | Binding affinity to HMOX2 cysteine residue in human 786-O cell soluble proteomic lysate at 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis | Homo sapiens | 3 | assay format | Scientific Literature | ||
11. | ALA4232638 | B | Binding affinity to NNMT C115 residue in human 786-O cell soluble proteomic lysate at 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
12. | ALA4232639 | B | Binding affinity to NNMT C75 residue in human 786-O cell soluble proteomic lysate at 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
13. | ALA4232640 | B | Binding affinity to NNMT C50 residue in human 786-O cell soluble proteomic lysate at 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
14. | ALA4232641 | B | Binding affinity to GSTO1 cysteine residue in human 786-O cell soluble proteomic lysate at 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
15. | ALA4232642 | B | Binding affinity to CKAP4 cysteine residue in human 786-O cell soluble proteomic lysate at 5 uM incubated for 1 hr followed by cell lysis by IA-alkyne probe based isoTOP-ABPP analysis | Homo sapiens | 2 | assay format | Scientific Literature |