# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4234459 | Binding | Inhibition of tyrosinase in mouse B16-F1 cell lysates assessed as reduction in melanin production at 25 uM using L-DOPA as substrate incubated for 30 mins followed by substrate addition measured after 30 mins | Mus musculus | 7 | ALA4229457 | single protein format | Scientific Literature | |
2. | ALA4234460 | ADME | Cytotoxicity against mouse B16-F1 cells assessed as reduction in cell viability at 25 uM after 72 hrs by MTT assay | Mus musculus | 6 | ALA4229457 | cell-based format | Scientific Literature | |
3. | ALA4234461 | Functional | Inhibition of alpha-MSH-stimulated melanogenesis in mouse B16-F1 cells assessed as reduction in intracellular melanin production at 25 uM after 72 hrs relative to control | Mus musculus | 7 | ALA4229457 | cell-based format | Scientific Literature | |
4. | ALA4234462 | Binding | Inhibition of adenylate cyclase in alpha-MSH-stimulated mouse B16-F1 cells assessed as reduction in cAMP-CREB signaling pathway-mediated TYR expression level at 25 uM after 48 to 72 hrs by Western blot analysis | Mus musculus | 6 | ALA4229457 | cell-based format | Scientific Literature | |
5. | ALA4234463 | Binding | Inhibition of adenylate cyclase in alpha-MSH-stimulated mouse B16-F1 cells assessed as reduction in cAMP-CREB signaling pathway-mediated DCT expression level at 25 uM after 48 to 72 hrs by Western blot analysis | Mus musculus | 6 | ALA4229457 | cell-based format | Scientific Literature |