#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4231159	B	Inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation at 3 to 300 uM pretreated for 30 mins followed by biotin-labelled ubiquitin addition and measured after 30 mins by Western blot analysis	Homo sapiens	1	ALA4229347	assay format	Scientific Literature
2.	ALA4231162	B	Irreversible inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation at 10 uM pretreated for 60 mins followed by 2.5 uM biotin-labelled ubiquitin addition for 30 mins and subsequent 5 uM biotin-labelled ubiquitin addition by Western blot analysis	Homo sapiens	1	ALA4229347	assay format	Scientific Literature
3.	ALA4231163	B	Irreversible inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation at 10 uM pretreated for 60 mins followed by 2.5 uM biotin-labelled ubiquitin addition for 30 mins and subsequent 5 uM biotin-labelled ubiquitin addition measured after 30 mins by Western blot analysis	Homo sapiens	1	ALA4229347	assay format	Scientific Literature
4.	ALA4231166	B	Inhibition of E1 in human MCF7 cells assessed as increase in p53 levels at 30 uM measured after 24 hrs by Western blot analysis	Homo sapiens	1	ALA4229347	cell-based format	Scientific Literature
5.	ALA4231168	F	Growth inhibition of human MCF7 cells after 72 hrs by alamar blue assay	Homo sapiens	2	ALA4229347	cell-based format	Scientific Literature