| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4231159 | B | Inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation at 3 to 300 uM pretreated for 30 mins followed by biotin-labelled ubiquitin addition and measured after 30 mins by Western blot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 2. | ALA4231162 | B | Irreversible inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation at 10 uM pretreated for 60 mins followed by 2.5 uM biotin-labelled ubiquitin addition for 30 mins and subsequent 5 uM biotin-labelled ubiquitin addition by Western blot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 3. | ALA4231163 | B | Irreversible inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation at 10 uM pretreated for 60 mins followed by 2.5 uM biotin-labelled ubiquitin addition for 30 mins and subsequent 5 uM biotin-labelled ubiquitin addition measured after 30 mins by Western blot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 4. | ALA4231166 | B | Inhibition of E1 in human MCF7 cells assessed as increase in p53 levels at 30 uM measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4231168 | F | Growth inhibition of human MCF7 cells after 72 hrs by alamar blue assay | Homo sapiens | 2 | cell-based format | Scientific Literature |
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