| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4231157 | B | Inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation up to 1000 uM pretreated for 30 mins followed by biotin-labelled ubiquitin addition and measured after 30 mins by Western blot analysis | Homo sapiens | 2 | ALA4229347 | assay format | Scientific Literature | |
| 2. | ALA4231160 | B | Inhibition of E2 (unknown origin) assessed as reduction in biotin-labelled ubiquitin-E2 conjugate formation at 10 to 1000 uM pretreated for 30 mins followed by biotin-labelled ubiquitin addition and measured after 60 mins in presence of E1 by Western blot analysis | Homo sapiens | 2 | ALA4229347 | single protein format | Scientific Literature |
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