| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4231611 | B | Inhibition of recombinant human 5-LO expressed in Escherichia coli BL21 incubated for 15 mins prior to prewarming for 30 secs followed by CaCl2+ and arachidonic acid addition and measured after 10 mins by RP-HPLC analysis | Homo sapiens | 4 | ALA4229367 | assay format | Scientific Literature | |
| 2. | ALA4231612 | B | Inhibition of FLAP in A23187-stimulated human neutrophils assessed as remaining enzyme activity by measuring 5-LO product formation at 0.1 uM preincubated for 15 mins followed by A23187 and arachidonic acid addition and measured after 10 mins by UV based RP-HPLC analysis (Rvb = 100%) | Homo sapiens | 34 | ALA4229367 | single protein format | Scientific Literature | |
| 3. | ALA4231613 | B | Inhibition of FLAP in A23187-stimulated human neutrophils assessed as remaining enzyme activity by measuring 5-LO product formation at 1 uM preincubated for 15 mins followed by A23187 and arachidonic acid addition and measured after 10 mins by UV based RP-HPLC analysis (Rvb = 100%) | Homo sapiens | 34 | ALA4229367 | single protein format | Scientific Literature | |
| 4. | ALA4231614 | B | Inhibition of FLAP in A23187-stimulated human neutrophils assessed as reduction in 5-LO product formation preincubated for 15 mins followed by A23187 and arachidonic acid addition and measured after 10 mins by UV based RP-HPLC analysis | Homo sapiens | 36 | ALA4229367 | single protein format | Scientific Literature | |
| 5. | ALA4231620 | B | Inhibition of mPGES1 in IL-1beta-stimulated human A549 cell microsomal fractions assessed as reduction in PGE2 production preincubated for 15 mins followed by PGH2 substrate addition and measured after 1 min by RP-HPLC analysis relative to control | Homo sapiens | 2 | ALA4229367 | assay format | Scientific Literature | |
| 6. | ALA4231621 | B | Inhibition of mPGES1 in IL-1beta-stimulated human A549 cell microsomal fractions assessed as reduction in PGE2 production at 3 uM preincubated for 15 mins followed by PGH2 substrate addition and measured after 1 min by RP-HPLC analysis relative to control | Homo sapiens | 19 | ALA4229367 | assay format | Scientific Literature | |
| 7. | ALA4231623 | B | Inhibition of human LTC4 synthase expressed in HEK293 cell microsomes preincubated for 10 mins followed by LTA4-methyl ester addition and measured after 10 mins by UPLC-MS/MS analysis | Homo sapiens | 2 | ALA4229367 | assay format | Scientific Literature |
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