#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4235563	B	Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells co-expressing pGL3-Elam.luc assessed as Pam3CSK4-stimulated NF-kappaB activation at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 5 hrs by luciferase reporter gene assay relative to control	Homo sapiens	2		cell-based format	Scientific Literature
2.	ALA4235564	B	Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells co-expressing pGL3-Elam.luc assessed as inhibition of Pam3CSK4-stimulated NF-kappaB activation preincubated for 1 hr followed by Pam3CSK4 stimulation for 5 hrs by luciferase reporter gene assay	Homo sapiens	2		cell-based format	Scientific Literature
3.	ALA4235565	B	Antagonist activity at FLAG-tagged human TLR2/TLR6 expressed in HEK293 cells co-expressing pGL3-Elam.luc assessed as inhibition of Pam2CSK4-stimulated NF-kappaB activation preincubated for 1 hr followed by Pam2CSK4 stimulation for 5 hrs by luciferase reporter gene assay	Homo sapiens	2		cell-based format	Scientific Literature
4.	ALA4235566	B	Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated MAPK p38 phosphorylation at Thr180/Tyr182 residues at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 35 mins by immunoblot analysis	Homo sapiens	2		cell-based format	Scientific Literature
5.	ALA4235567	B	Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated JNK phosphorylation at Thr183/Tyr185 residues at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 35 mins by immunoblot analysis	Homo sapiens	2		cell-based format	Scientific Literature
6.	ALA4235570	B	Antagonist activity at FLAG-tagged human TLR2/TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated IkBalpha degradation at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 35 mins by immunoblot analysis	Homo sapiens	2		cell-based format	Scientific Literature
7.	ALA4235571	B	Antagonist activity at TLR2/TLR1 in human THP1 cells assessed as inhibition of Pam3CSK4-stimulated non-canonical NF-kB pathway by measuring reduction in p52 level at 50 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 2 hrs by immunoblot analysis	Homo sapiens	2		cell-based format	Scientific Literature
8.	ALA4235572	B	Antagonist activity at TLR2/TLR1 in human PBMC assessed as inhibition of Pam3CSK4-stimulated TNFalpha production at 50 uM preincubated with cells followed by overnight Pam3CSK4 stimulation by ELISA relative to control	Homo sapiens	2		cell-based format	Scientific Literature
9.	ALA4235573	B	Antagonist activity at TLR2/TLR1 in mouse BMDM assessed as inhibition of Pam3CSK4-stimulated TNFalpha production by measuring TNFalpha level at 25 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 11 hrs by ELISA relative to control	Mus musculus	2		cell-based format	Scientific Literature
10.	ALA4235574	B	Antagonist activity at TLR2/TLR1 in human PBMC assessed as inhibition of Pam3CSK4-stimulated IL-10 production by measuring IL-10 level at 100 uM preincubated with cells followed by overnight Pam3CSK4 stimulation by ELISA (Rvb = 794 pg/ml)	Homo sapiens	1		cell-based format	Scientific Literature
11.	ALA4235576	B	Antagonist activity at TLR4 in mouse BMDM assessed as inhibition of LPS-stimulated TNFalpha production at 25 uM preincubated for 1 hr followed by LPS stimulation for 11 hrs by ELISA	Mus musculus	2		cell-based format	Scientific Literature
12.	ALA4235577	B	Antagonist activity at TLR7/TLR8 in mouse BMDM assessed as inhibition of R848-stimulated TNFalpha production at 25 uM preincubated for 1 hr followed by R848 stimulation for 11 hrs by ELISA	Mus musculus	2		cell-based format	Scientific Literature
13.	ALA4235578	B	Antagonist activity at TLR3 in mouse BMDM assessed as inhibition of poly I:C-stimulated TNFalpha production at 25 uM preincubated for 1 hr followed by poly I:C stimulation for 11 hrs by ELISA	Mus musculus	2		cell-based format	Scientific Literature
14.	ALA4235579	A	Cytotoxicity against HEK293 cells at 100 uM after overnight incubation by CCK8 assay	Homo sapiens	2		cell-based format	Scientific Literature
15.	ALA4235580	A	Cytotoxicity against mouse BMDM at 100 uM after overnight incubation by CCK8 assay	Mus musculus	2		cell-based format	Scientific Literature
16.	ALA4235581	A	Cytotoxicity against human THP1 cells at 100 uM after overnight incubation by CCK8 assay	Homo sapiens	2		cell-based format	Scientific Literature
17.	ALA4235582	B	Antagonist activity at TLR2/TLR1 in mouse BMDM assessed as inhibition of Rhodamine-labeled Pam3CSK4-stimulated receptor internalization at 50 uM preincubated for 1 hr followed by Rhodamine-Pam3CSK4 stimulation for 20 mins by DAPI staining based phase contrast fluorescence microscopic method	Mus musculus	2		cell-based format	Scientific Literature
18.	ALA4235584	B	Antagonist activity at TLR2/TLR1 in mouse BMDM assessed as Rhodamine-labeled Pam3CSK4-stimulated receptor internalization at 50 uM preincubated for 1 hr followed by Rhodamine-Pam3CSK4 stimulation for 20 mins by FACS analysis relative to control	Mus musculus	2		cell-based format	Scientific Literature
19.	ALA4235585	B	Antagonist activity at FLAG-tagged human TLR2/YFP-tagged TLR1 expressed in HEK293 cells assessed as inhibition of Pam3CSK4-stimulated TLR1/TLR2 heterodimerization at 25 uM preincubated for 1 hr followed by Pam3CSK4 stimulation for 15 mins by immunoblot analysis	Homo sapiens	2		cell-based format	Scientific Literature