# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4234621 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha2beta2gamma3 expressed in Escherichia coli BL21 cells using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
2. | ALA4234622 | A | Half life in rat hepatocytes | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
3. | ALA4234623 | A | Protein binding in monkey plasma assessed as unbound fraction | Macaca mulatta | 2 | cell-free format | Scientific Literature | ||
4. | ALA4234624 | A | Protein binding in human plasma assessed as unbound fraction | Homo sapiens | 2 | cell-free format | Scientific Literature | ||
5. | ALA4234625 | A | Protein binding in rat plasma assessed as unbound fraction | Rattus norvegicus | 2 | cell-free format | Scientific Literature | ||
6. | ALA4234626 | A | Intrinsic clearance in human primary hepatocytes assessed per million cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
7. | ALA4234627 | A | Half life in human primary hepatocytes at 10 uM | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
8. | ALA4234628 | B | Activation of recombinant AMPKbeta1 in cryopreserved human hepatocytes assessed as reduction in 14C-2-acetic acid incorporation after 1 hr by scintillation counting analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
9. | ALA4234629 | B | Activation of AMPK in cryopreserved human hepatocytes assessed as increase in phosphorylation of acetyl-CoA carboxylase at Ser79 residue after 1 hr by Meso Scale Discovery assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
10. | ALA4234630 | B | Binding affinity to Bap-tagged human AMPKalpha1beta1gamma1 expressed in Escherichia coli BL21 cells by SPR analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
11. | ALA4234631 | B | Binding affinity to Bap-tagged human AMPKalpha1beta1gamma1 expressed in Escherichia coli BL21 cells assessed as dissociation rate constant by SPR analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
12. | ALA4234632 | B | Binding affinity to Bap-tagged human AMPKalpha1beta1gamma1 expressed in Escherichia coli BL21 cells assessed as association rate constant by SPR analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
13. | ALA4234633 | B | Binding affinity to Bap-tagged human AMPKalpha1beta1gamma1 expressed in Escherichia coli BL21 cells assessed as dissociation half life by SPR analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
14. | ALA4234634 | A | Apparent passive permeability of the compound in RRCK cells by FACS analysis | Canis lupus familiaris | 6 | cell-based format | Scientific Literature | ||
15. | ALA4234635 | P | Lipophilicity, log D of the compound at pH 7.4 at 0.52 mg after 1 hr by shake-flask method | 6 | small-molecule physicochemical format | Scientific Literature | |||
16. | ALA4234636 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha2beta2gamma3 expressed in Escherichia coli BL21 cells at 1 nM using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay relative to AMP control | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
17. | ALA4234637 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha2beta2gamma1 expressed in Escherichia coli BL21 cells at 1 nM using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay relative to AMP control | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
18. | ALA4234638 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha1beta2gamma1 expressed in Escherichia coli BL21 cells at 0.5 nM using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay relative to AMP control | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
19. | ALA4234639 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha2beta1gamma1 expressed in Escherichia coli BL21 cells at 2 nM using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay relative to AMP control | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
20. | ALA4234640 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha1beta1gamma1 expressed in Escherichia coli BL21 cells at 0.25 nM using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay relative to AMP control | Homo sapiens | 6 | cell-based format | Scientific Literature |