| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4231612 | B | Inhibition of FLAP in A23187-stimulated human neutrophils assessed as remaining enzyme activity by measuring 5-LO product formation at 0.1 uM preincubated for 15 mins followed by A23187 and arachidonic acid addition and measured after 10 mins by UV based RP-HPLC analysis (Rvb = 100%) | Homo sapiens | 34 | ALA4229367 | single protein format | Scientific Literature | |
| 2. | ALA4231613 | B | Inhibition of FLAP in A23187-stimulated human neutrophils assessed as remaining enzyme activity by measuring 5-LO product formation at 1 uM preincubated for 15 mins followed by A23187 and arachidonic acid addition and measured after 10 mins by UV based RP-HPLC analysis (Rvb = 100%) | Homo sapiens | 34 | ALA4229367 | single protein format | Scientific Literature | |
| 3. | ALA4231614 | B | Inhibition of FLAP in A23187-stimulated human neutrophils assessed as reduction in 5-LO product formation preincubated for 15 mins followed by A23187 and arachidonic acid addition and measured after 10 mins by UV based RP-HPLC analysis | Homo sapiens | 36 | ALA4229367 | single protein format | Scientific Literature | |
| 4. | ALA4231615 | B | Inhibition of FLAP in A23187-stimulated human monocytes assessed as reduction in 5-LO product formation preincubated for 15 mins followed by A23187 and arachidonic acid addition and measured after 10 mins by UV based RP-HPLC analysis | Homo sapiens | 1 | ALA4229367 | cell-based format | Scientific Literature | |
| 5. | ALA4231616 | B | Inhibition of 5-LO in A23187-stimulated human neutrophils assessed as reduction in 5-LO product formation preincubated for 15 mins followed by A23187 and 10 uM arachidonic acid addition and measured after 10 mins by UV based RP-HPLC analysis | Homo sapiens | 1 | ALA4229367 | single protein format | Scientific Literature | |
| 6. | ALA4231617 | B | Inhibition of LTC4 synthase in LPS/fMLP-stimulated human monocytes assessed as decrease in cys-LT formation pre-incubated with LPS for 30 mins and later stimulated with fMLP for 10 mins by ELISA | Homo sapiens | 1 | ALA4229367 | cell-based format | Scientific Literature | |
| 7. | ALA4231618 | B | Inhibition of recombinant human 5-LO expressed in Escherichia coli BL21 at 10 uM incubated for 15 mins prior to prewarming for 30 secs followed by CaCl2+ and arachidonic acid addition and measured after 10 mins by RP-HPLC analysis | Homo sapiens | 1 | ALA4229367 | assay format | Scientific Literature | |
| 8. | ALA4231621 | B | Inhibition of mPGES1 in IL-1beta-stimulated human A549 cell microsomal fractions assessed as reduction in PGE2 production at 3 uM preincubated for 15 mins followed by PGH2 substrate addition and measured after 1 min by RP-HPLC analysis relative to control | Homo sapiens | 19 | ALA4229367 | assay format | Scientific Literature |
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