| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4272239 | Binding | Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins | Mus musculus | 17 | ALA4270537 | single protein format | Scientific Literature | |
| 2. | ALA4272240 | Binding | Inhibition of recombinant human full length GST-tagged wild type BRAF expressed in baculovirus expression system using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins | Homo sapiens | 17 | ALA4270537 | assay format | Scientific Literature | |
| 3. | ALA4272241 | Functional | Antiproliferative activity against human A375 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 4. | ALA4272242 | Functional | Antiproliferative activity against human WM266.4 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 5. | ALA4272243 | Functional | Antiproliferative activity against human WM1361 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 6. | ALA4272244 | Functional | Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 7. | ALA4272245 | Functional | Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 8. | ALA4272246 | Binding | Inhibition of recombinant human GST-tagged c-RAF (306 to 648 residues) Y340D/Y341D mutant expressed in baculovirus expression system using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins | Homo sapiens | 3 | ALA4270537 | assay format | Scientific Literature | |
| 9. | ALA4272247 | ADME | Cytotoxicity against HEK293T cells assessed as decrease in cell viability after 48 hrs by MTT assay | Homo sapiens | 3 | ALA4270537 | cell-based format | Scientific Literature | |
| 10. | ALA4272248 | ADME | Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 48 hrs by MTT assay | Homo sapiens | 3 | ALA4270537 | cell-based format | Scientific Literature | |
| 11. | ALA4272249 | Binding | Inhibition of MEK1/2 (unknown origin) | Homo sapiens | 3 | ALA4270537 | protein format | Scientific Literature | |
| 12. | ALA4272250 | Binding | Inhibition of N-terminal flag-tagged JNK1 (unknown origin) expressed in baculovirus expression system after 60 mins in presence of [gamma-32P]ATP by topcount scintillation method | Homo sapiens | 3 | ALA4270537 | assay format | Scientific Literature | |
| 13. | ALA4272251 | Binding | Inhibition of N-terminal flag-tagged p38alpha (unknown origin) expressed in baculovirus expression system using MBP as substrate preincubated for 5 mins followed by substrate addition measured after 60 mins in presence of [gamma-32P]ATP by topcount scintillation method | Homo sapiens | 3 | ALA4270537 | assay format | Scientific Literature | |
| 14. | ALA4272252 | Functional | Induction of apoptosis in human A375 cells assessed as viable cells at 2 uM after 24 hrs by Annexin V-FITC/PI staining based flow cytometry (Rvb = 89.4%) | Homo sapiens | 1 | ALA4270537 | cell-based format | Scientific Literature | |
| 15. | ALA4272253 | Functional | Induction of apoptosis in human A375 cells assessed as early apoptotic cells at 2 uM after 24 hrs by Annexin V-FITC/PI staining based flow cytometry (Rvb = 5.42%) | Homo sapiens | 1 | ALA4270537 | cell-based format | Scientific Literature | |
| 16. | ALA4272254 | Functional | Induction of apoptosis in human A375 cells assessed as late apoptotic cells at 2 uM after 24 hrs by Annexin V-FITC/PI staining based flow cytometry (Rvb = 3.61%) | Homo sapiens | 1 | ALA4270537 | cell-based format | Scientific Literature | |
| 17. | ALA4272255 | Functional | Induction of apoptosis in human A375 cells assessed as necrotic cells at 2 uM after 24 hrs by Annexin V-FITC/PI staining based flow cytometry (Rvb = 1.55%) | Homo sapiens | 1 | ALA4270537 | cell-based format | Scientific Literature | |
| 18. | ALA4272256 | Functional | Induction of apoptosis in human A375 cells assessed as viable cells at 2 uM after 48 hrs by Annexin V-FITC/PI staining based flow cytometry (Rvb = 89.4%) | Homo sapiens | 1 | ALA4270537 | cell-based format | Scientific Literature | |
| 19. | ALA4272257 | Functional | Induction of apoptosis in human A375 cells assessed as early apoptotic cells at 2 uM after 48 hrs by Annexin V-FITC/PI staining based flow cytometry (Rvb = 5.42%) | Homo sapiens | 1 | ALA4270537 | cell-based format | Scientific Literature | |
| 20. | ALA4272258 | Functional | Induction of apoptosis in human A375 cells assessed as late apoptotic cells at 2 uM after 48 hrs by Annexin V-FITC/PI staining based flow cytometry (Rvb = 3.61%) | Homo sapiens | 1 | ALA4270537 | cell-based format | Scientific Literature |
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