# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4256413 | Binding | Irreversible inhibition of human leukocyte elastase assessed as ratio of Kinact to Ki using MeO-Suc-Ala-Ala-Pro-Val-pNA as substrate incubated over 60 mins by spectrophotometric method | Homo sapiens | 4 | ALA4251738 | single protein format | Scientific Literature | |
2. | ALA4256414 | Binding | Inhibition of porcine pancreatic elastase assessed as ratio of Kinact to Ki using MeO-Suc-Ala-Ala-Pro-Val-pNA as substrate incubated over 60 mins by spectrophotometric method | Sus scrofa | 2 | ALA4251738 | protein format | Scientific Literature | |
3. | ALA4256415 | Binding | Inhibition of bovine chymotrypsin assessed as ratio of Kinact to Ki using Suc-Ala-Ala-Pro-Phe-pNA as substrate incubated over 60 mins by spectrophotometric method | Bos taurus | 1 | ALA4251738 | assay format | Scientific Literature | |
4. | ALA4256416 | Binding | Inhibition of bovine factor 10a assessed as product formation at 1 uM using Boc-Ile-Glu-Gly-Arg-AMC as substrate incubated over 60 mins by fluorimetric method | Bos taurus | 3 | ALA4251738 | single protein format | Scientific Literature | |
5. | ALA4256417 | Binding | Inhibition of human thrombin assessed as product formation at 1 uM using Z-Gly-Gly-Arg-AMC as substrate incubated over 60 mins by fluorimetric method | Homo sapiens | 3 | ALA4251738 | single protein format | Scientific Literature | |
6. | ALA4256418 | Binding | Inhibition of bovine trypsin assessed as product formation at 1 uM using Suc-Ala-Ala-Pro-Arg-pNA as substrate incubated over 60 mins by spectrophotometric method | Bos taurus | 3 | ALA4251738 | assay format | Scientific Literature | |
7. | ALA4256419 | Binding | Inhibition of human cathepsin B assessed as product formation at 1 uM using Z-Arg-Arg-pNA as substrate incubated over 60 mins by spectrophotometric method | Homo sapiens | 3 | ALA4251738 | single protein format | Scientific Literature | |
8. | ALA4256420 | Binding | Inhibition of human cathepsin L assessed as product formation at 1 uM using Z-Phe-Arg-pNA as substrate incubated over 60 mins by spectrophotometric method | Homo sapiens | 3 | ALA4251738 | single protein format | Scientific Literature | |
9. | ALA4256427 | Binding | Irreversible inhibition of human leukocyte elastase at 50 nM using MeO-Suc-Ala-Ala-Pro-Val-pNA as substrate incubated for 30 mins followed by substrate addition | Homo sapiens | 1 | ALA4251738 | single protein format | Scientific Literature | |
10. | ALA4256428 | Binding | Binding affinity to human leukocyte elastase at 2.5 uM after 20 mins by in gel fluorescence detection based SDS-PAGE analysis | Homo sapiens | 1 | ALA4251738 | single protein format | Scientific Literature | |
11. | ALA4256429 | Binding | Binding affinity to human leukocyte elastase at 160 ng after 20 mins by in gel fluorescence detection based SDS-PAGE analysis | Homo sapiens | 1 | ALA4251738 | single protein format | Scientific Literature | |
12. | ALA4256430 | Binding | Binding affinity to human leukocyte elastase at 2.5 uM after 20 mins by Coomassie brilliant blue G-250 staining based assay | Homo sapiens | 1 | ALA4251738 | single protein format | Scientific Literature | |
13. | ALA4256431 | Binding | Binding affinity to human leukocyte elastase at 2.5 uM incubated for 20 mins followed by HEK cell lysate addition by in gel fluorescence detection based SDS-PAGE analysis | Homo sapiens | 1 | ALA4251738 | assay format | Scientific Literature | |
14. | ALA4256432 | Binding | Binding affinity to human leukocyte elastase at 2.5 uM incubated for 20 mins post HEK cell lysate addition by in gel fluorescence detection based SDS-PAGE analysis | Homo sapiens | 1 | ALA4251738 | assay format | Scientific Literature | |
15. | ALA4256433 | Binding | Binding affinity to human leukocyte elastase at 2.5 uM incubated for 20 mins followed by HEK cell lysate addition by Coomassie brilliant blue G-250 staining based assay | Homo sapiens | 1 | ALA4251738 | assay format | Scientific Literature | |
16. | ALA4256434 | Binding | Binding affinity to human leukocyte elastase at 2.5 uM incubated for 20 mins post HEK cell lysate addition by Coomassie brilliant blue G-250 staining based assay | Homo sapiens | 1 | ALA4251738 | assay format | Scientific Literature | |
17. | ALA4256435 | Binding | Inhibition of human leukocyte elastase at 2.5 uM preincubated with sivelestat for 5 mins followed by compound addition measured after 20 mins in presence of sivelestat by in gel fluorescence detection based SDS-PAGE analysis | Homo sapiens | 1 | ALA4251738 | single protein format | Scientific Literature | |
18. | ALA4256436 | Binding | Inhibition of human leukocyte elastase at 2.5 uM preincubated with sivelestat for 5 mins followed by compound addition measured after 20 mins in presence of sivelestat by Coomassie brilliant blue G-250 staining based assay | Homo sapiens | 1 | ALA4251738 | single protein format | Scientific Literature |