Inhibition of Zymomonas mobilis pyruvate decarboxylase incubated for 3 mins using pyruvate as substrate in presence of TPP by spectrophotometric analysis
Inhibition of TPP-dependent porcine pyruvate dehydrogenase E1 component at 250 uM preincubated for 30 mins followed by substrate addition using pyruvate as substrate in presence of TPP by DCPIP dye based microplate reader analysis
Inhibition of porcine PDHc E1-subunit assessed as DCPIP reduction at 250 uM preincubated for 30 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis
Inhibition of Escherichia coli OGDHc E1 subunit assessed as DCPIP reduction at 250 uM preincubated for 60 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis
Inhibition of Saccharomyces cerevisiae pyruvate decarboxylase assessed as DCPIP reduction at 1.5 mM preincubated for 60 mins followed by pyruvate addition using 0.3 mM ThDP as substrate by microplate reader analysis
Inhibition of Aerococcus viridans pyruvate oxidase at 250 uM preincubated for 30 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis