# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4255910 | Binding | Inhibition of STAT3 DNA binding activity in mouse NIH/3T3 nuclear extract preincubated for 30 mins followed by [32P]hSIE addition measured after 30 mins by electrophoretic mobility shift assay | Mus musculus | 82 | ALA4251703 | single protein format | Scientific Literature | |
2. | ALA4255915 | Binding | Inhibition of STAT3 DNA binding activity in human MDA-MB-468 nuclear extract at 5 uM after 3 hrs by electrophoretic mobility shift assay | Homo sapiens | 1 | ALA4251703 | single protein format | Scientific Literature | |
3. | ALA4255917 | Binding | Inhibition of STAT3 in human MDA-MB-231 cells assessed as reduction in p-STAT3(Y705) level at 10 uM after 1 hr by immunoblot analysis | Homo sapiens | 4 | ALA4251703 | cell-based format | Scientific Literature | |
4. | ALA4255923 | Binding | Inhibition of JAK2 phosphorylation in human MDA-MB-231 cells at 5 uM after 24 hrs by immunoblot analysis | Homo sapiens | 2 | ALA4251703 | cell-based format | Scientific Literature | |
5. | ALA4255924 | Binding | Inhibition of SRC phosphorylation in human MDA-MB-231 cells at 5 uM after 24 hrs by immunoblot analysis | Homo sapiens | 2 | ALA4251703 | cell-based format | Scientific Literature | |
6. | ALA4255925 | Binding | Inhibition of ERK1/2 phosphorylation in human MDA-MB-231 cells at 5 uM after 24 hrs by immunoblot analysis | Homo sapiens | 2 | ALA4251703 | cell-based format | Scientific Literature | |
7. | ALA4255926 | Functional | Cytotoxicity against human MDA-MB-231 cells harboring active STAT3 assessed as decrease in cell viability up to 10 uM after 72 hrs by CyQuant dye based assay | Homo sapiens | 6 | ALA4251703 | cell-based format | Scientific Literature | |
8. | ALA4255927 | Functional | Cytotoxicity against human MDA-MB-468 cells harboring active STAT3 assessed as decrease in cell viability up to 10 uM after 72 hrs by CyQuant dye based assay | Homo sapiens | 6 | ALA4251703 | cell-based format | Scientific Literature | |
9. | ALA4255929 | Functional | Cytotoxicity against human MCF7 cells assessed as decrease in cell viability up to 10 uM after 72 hrs by CyQuant dye based assay | Homo sapiens | 6 | ALA4251703 | cell-based format | Scientific Literature | |
10. | ALA4255934 | Functional | Anticlonogenic activity against human MDA-MB-231 cells up to 3 uM after 4 hrs by crystal violet staining based assay | Homo sapiens | 4 | ALA4251703 | cell-based format | Scientific Literature | |
11. | ALA4255935 | Functional | Antimigratory activity against human MDA-MB-231 cells at 8 uM after 22 to 33 hrs by inverted fluorescence microscope | Homo sapiens | 4 | ALA4251703 | cell-based format | Scientific Literature |