# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4275884 | Binding | Binding affinity to bovine articular cartilage aggrecan at 500 uM by SPR analysis | Bos taurus | 12 | ALA4270645 | single protein format | Scientific Literature | |
2. | ALA4275885 | Binding | Binding affinity to bovine articular cartilage aggrecan by SPR analysis | Bos taurus | 13 | ALA4270645 | single protein format | Scientific Literature | |
3. | ALA4275886 | ADME | Antiproliferative activity against human HEMC-SS cells treated for 24 hrs under normoxic condition followed by compound wash-out and incubated under normoxic condition for 48 hrs by alamar blue assay | Homo sapiens | 14 | ALA4270645 | cell-based format | Scientific Literature | |
4. | ALA4275887 | Functional | Antiproliferative activity against human HEMC-SS cells treated for 24 hrs under hypoxic condition followed by compound wash-out and incubated under normoxic condition for 48 hrs by alamar blue assay | Homo sapiens | 14 | ALA4270645 | cell-based format | Scientific Literature | |
5. | ALA4275888 | Functional | Selectivity index, ratio of IC50 for human HEMC-SS cells under normoxic condition to IC50 for human HEMC-SS cells under hypoxic condition | Homo sapiens | 14 | ALA4270645 | cell-based format | Scientific Literature | |
6. | ALA4275889 | Physicochemical | Chemical stability in pH 7.4 sodium phosphate buffer assessed as drug metabolism at 17 uM at 37 degC after 24 hrs by RP-HPLC analysis | 1 | ALA4270645 | small-molecule physicochemical format | Scientific Literature | ||
7. | ALA4275890 | ADME | Stability in rabbit plasma at 37 degC after 24 hrs by 31P NMR analysis | Oryctolagus cuniculus | 1 | ALA4270645 | cell-free format | Scientific Literature | |
8. | ALA4275891 | Physicochemical | Chemical stability in 3 equiv sodium dithionite/CH3CN,/cacodylate buffer at pH 7.4 at 37 degC after 24 hrs by 31P NMR analysis | 1 | ALA4270645 | small-molecule physicochemical format | Scientific Literature | ||
9. | ALA4275892 | Physicochemical | Prodrug activation in 3 equiv sodium dithionite/CH3CN,/cacodylate buffer at pH 7.4 assessed as bis(2-chloroethyl)phosphoramidate anion formation by measuring half life at 37 degC by 31P NMR analysis | 1 | ALA4270645 | small-molecule physicochemical format | Scientific Literature | ||
10. | ALA4275893 | Physicochemical | Prodrug activation in 3 equiv sodium dithionite/CH3CN,/cacodylate buffer at pH 7.4 assessed as bis(2-chloroethyl)phosphoramidate anion formation at 37 degC up to 8 mins by 31P NMR analysis | 1 | ALA4270645 | small-molecule physicochemical format | Scientific Literature | ||
11. | ALA4275894 | ADME | Prodrug activation in pH 7.4 PBS buffer assessed as 15 to 25 ug/ml Escherichia coli nitroreductase-mediated bis(2-chloroethyl)phosphoramidate anion formation by measuring half life at 37 degC in presence of NADPH by RP-HPLC analysis | Escherichia coli | 1 | ALA4270645 | assay format | Scientific Literature | |
12. | ALA4275897 | ADME | Prodrug activation in pH 7.4 PBS buffer assessed as 15 to 25 ug/ml Escherichia coli nitroreductase-mediated bis(2-chloroethyl)phosphoramidate anion formation at 37 degC after 3 to 5 mins in presence of NADPH by RP-HPLC analysis | Escherichia coli | 1 | ALA4270645 | assay format | Scientific Literature |