#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4267589	F	Agonist activity at recombinant human GPR40 expressed in HEK293 cells assessed as increase in intracellular calcium flux at 10 uM measured for 120 secs by calcium-5 dye based assay relative to 30 uM linolenic acid	Homo sapiens	26	ALA4265918	cell-based format	Scientific Literature
2.	ALA4267590	F	Agonist activity at recombinant human GPR40 expressed in HEK293 cells assessed as increase in intracellular calcium flux measured for 120 secs by calcium-5 dye based assay	Homo sapiens	21	ALA4265918	cell-based format	Scientific Literature
3.	ALA4267591	A	Stability of the compound in rat liver microsomes assessed as parent compound remaining at 10 uM after 1 hr	Rattus norvegicus	3	ALA4265918	microsome format	Scientific Literature
4.	ALA4267592	F	Agonist activity at recombinant human GPR40 expressed in HEK293 cells assessed as increase in intracellular calcium flux measured for 120 secs in presence of GPR40 antagonist GW-100 by calcium-5 dye based assay	Homo sapiens	4	ALA4265918	cell-based format	Scientific Literature
5.	ALA4267593	F	Agonist activity at recombinant human GPR40 expressed in HEK293 cells assessed as phosphorylation of ERK1/2 at 10 after 20 mins by Western blot analysis	Homo sapiens	4	ALA4265918	cell-based format	Scientific Literature
6.	ALA4267594	F	Agonist activity at recombinant human GPR40 expressed in HEK293 cells assessed as phosphorylation of ERK1/2 at 10 after 20 mins in presence of GPR40 antagonist GW-100 by Western blot analysis	Homo sapiens	3	ALA4265918	cell-based format	Scientific Literature
7.	ALA4267595	F	Desensitization of recombinant human GPR40 expressed in HEK293 cells assessed as reduction in TAK-875-induced intracellular calcium flux at 10 uM pretreated for 10 mins followed by compound washout and subsequent TAK-875 stimulation by calcium-5 dye based assay	Homo sapiens	3	ALA4265918	cell-based format	Scientific Literature
8.	ALA4267596	F	Agonist activity at GPR40 in mouse MIN6 cells assessed as increase in glucose-stimulated insulin secretion at 10 uM measured after 2 hrs by ELISA relative to control	Mus musculus	4	ALA4265918	cell-based format	Scientific Literature
9.	ALA4267597	A	Effect on 2.5 mM glucose-induced insulin secretion in mouse MIN6 cells at 10 uM measured after 2 hrs by ELISA	Mus musculus	4	ALA4265918	cell-based format	Scientific Literature
10.	ALA4267599	F	Agonist activity at GPR40 in mouse MIN6 cells assessed as increase in glucose-stimulated insulin secretion at 10 uM measured after 2 hrs in presence of GPR40 antagonist GW-100 by ELISA	Mus musculus	4	ALA4265918	cell-based format	Scientific Literature