| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4273596 | B | Inhibition of mPGES-1 isolated from microsomes of interleukin-1 beta-stimulated human A549 cells using PGH2 as substrate preincubated for 15 mins followed by substrate addition measured after 1 min by RP-HPLC analysis | Homo sapiens | 28 | ALA4270576 | assay format | Scientific Literature | |
| 2. | ALA4273597 | B | Inhibition of recombinant human 5-LO expressed in Escherichia coli BL21 (DE3) using arachidonic acid as substrate preincubated for 10 mins followed by CaCl2 and substrate addition measured after 10 mins by RP-HPLC analysis | Homo sapiens | 11 | ALA4270576 | assay format | Scientific Literature | |
| 3. | ALA4273598 | B | Inhibition of 5-LO in human neutrophils using arachidonic acid as substrate assessed as reduction in 5-LO product formation preincubated for 15 mins followed by substrate addition measured after 10 mins in presence of Ca2+ ionophore A23187 by HPLC analysis | Homo sapiens | 28 | ALA4270576 | single protein format | Scientific Literature | |
| 4. | ALA4273599 | B | Inhibition of cPLA2alpha-mediated [3H]-arachidonic acid release in Ca2+ ionophore A23187-stimulated human neutrophils at 10 uM preincubated for 15 mins followed by Ca2+ ionophore A23187 stimulation measured after 5 mins by scintillation counting method | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 5. | ALA4273600 | B | Inhibition of nuclear localization of 5-LO (unknown origin) expressed in HEK293 cells at 10 uM preincubated for 10 mins followed by Ca2+ ionophore A23187 stimulation for 20 mins and measured after 1 hr by DAPI staining-based immunofluorescence microscopic analysis | Homo sapiens | 1 | ALA4270576 | cell-based format | Scientific Literature | |
| 6. | ALA4273601 | B | Inhibition of recombinant human 5-LO binding to FLAP expressed in HEK293 cells at 10 uM after 1 hr by DAPI staining-based immunofluorescence microscopic analysis | Homo sapiens | 1 | ALA4270576 | cell-based format | Scientific Literature | |
| 7. | ALA4273602 | B | Inhibition of 12-LO in Ca2+ ionophore A23187-stimulated human neutrophils using arachidonic acid as substrate assessed as suppression of 12-HETE formation after 10 mins | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 8. | ALA4273603 | B | Inhibition of 15-LO in Ca2+ ionophore A23187-stimulated human neutrophils using arachidonic acid as substrate assessed as suppression of 15-HETE formation after 10 mins | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 9. | ALA4273604 | B | Inhibition of 5-LO in human M1 macrophages derived from LPS/IFNgamma-polarized human monocyte derived macrophages assessed as effect on 5-HETE level at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 10. | ALA4273605 | B | Inhibition of 5-LO in human M1 macrophages derived from LPS/IFNgamma-polarized human monocyte derived macrophages assessed as effect on 5-HEPE level at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 11. | ALA4273606 | B | Inhibition of 5-LO in human M1 macrophages derived from LPS/IFNgamma-polarized human monocyte derived macrophages assessed as effect on t-LTB4 level at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 12. | ALA4273607 | B | Inhibition of 5-LO in human M1 macrophages derived from LPS/IFNgamma-polarized human monocyte derived macrophages assessed as effect on LTB4 level at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 13. | ALA4273608 | B | Inhibition of 5-LO in human M1 macrophages derived from LPS/IFNgamma-polarized human monocyte derived macrophages assessed as effect on 5,6-diHETE level at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 14. | ALA4273609 | B | Inhibition of mPGES-1 in LPS/IFNgamma-differentiated human M1 macrophages assessed as effect on PGE2 level at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 15. | ALA4273610 | A | Modulation of COX1/2-mediated PGF2a level in human M1 macrophages derived from LPS/IFNgamma-polarized human monocyte derived macrophages at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | protein format | Scientific Literature | |
| 16. | ALA4273611 | B | Modulation of 15-LO-mediated 17-HDHA level in human M2 macrophages derived from IL4-polarized human monocyte derived macrophages at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 17. | ALA4273612 | B | Modulation of 15-LO-mediated 7-HDHA level in human M2 macrophages derived from IL4-polarized human monocyte derived macrophages at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 18. | ALA4273613 | B | Modulation of 15-LO-mediated 15-HETE level in human M2 macrophages derived from IL4-polarized human monocyte derived macrophages at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 19. | ALA4273614 | B | Modulation of 15-LO-mediated 15-HEPE level in human M2 macrophages derived from IL4-polarized human monocyte derived macrophages at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature | |
| 20. | ALA4273615 | B | Modulation of 12-LO-mediated 14-HDHA level in human M2 macrophages derived from IL4-polarized human monocyte derived macrophages at 3 uM preincubated for 15 mins followed by Escherichia coli (O6:K2:H1) challenge for 90 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4270576 | single protein format | Scientific Literature |
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