# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4275246 | Binding | Inhibition of recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli using 4-NPA as substrate measured after 30 mins | Homo sapiens | 41 | ALA4270621 | assay format | Scientific Literature | |
2. | ALA4275247 | Binding | Inhibition of recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli using 4-NPA as substrate pretreated with substrate 15 mins followed by enzyme addition measured after 30 mins | Homo sapiens | 1 | ALA4270621 | assay format | Scientific Literature | |
3. | ALA4275248 | Binding | Inhibition of recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli using 4-NPA as substrate pretreated with substrate 15 mins in presence of DTT followed by enzyme addition measured after 30 mins | Homo sapiens | 1 | ALA4270621 | assay format | Scientific Literature | |
4. | ALA4275249 | Binding | Reversible inhibition of recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli at 20 uM using 4-NPA as substrate pretreated for 60 mins followed by 40 times compound dilution for 15 mins and subsequent substrate addition measured after 30 mins | Homo sapiens | 1 | ALA4270621 | assay format | Scientific Literature | |
5. | ALA4275250 | Binding | Reversible inhibition of recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli using 4-NPA as substrate pretreated up to 60 mins followed by substrate addition and measured after 30 mins | Homo sapiens | 1 | ALA4270621 | assay format | Scientific Literature | |
6. | ALA4275251 | Binding | Inhibition of FAAH in human U937 cells using [ethanolamine-1-3H]AEA as substrate pretreated for 30 mins followed by substrate addition and measured after 15 mins by liquid scintillation counting method | Homo sapiens | 2 | ALA4270621 | cell-based format | Scientific Literature | |
7. | ALA4275252 | Binding | Inhibition of recombinant human ABHD6 expressed in HEK293 cells using 2-OG as substrate pretreated for 30 mins followed by substrate addition and measured after 5 mins by liquid scintillation spectroscopy analysis | Homo sapiens | 2 | ALA4270621 | cell-based format | Scientific Literature | |
8. | ALA4275253 | Binding | Inhibition of recombinant human ABHD12 expressed in HEK293 cells using 2-OG as substrate pretreated for 30 mins followed by substrate addition and measured after 5 mins by liquid scintillation spectroscopy analysis | Homo sapiens | 2 | ALA4270621 | cell-based format | Scientific Literature | |
9. | ALA4275254 | Binding | Displacement of [3H]CP55940 from recombinant human CB1 expressed crude membranes after 90 mins by liquid scintillation counting method | Homo sapiens | 2 | ALA4270621 | assay format | Scientific Literature | |
10. | ALA4275255 | Binding | Displacement of [3H]CP55940 from recombinant human CB2 expressed crude membranes after 90 mins by liquid scintillation counting method | Homo sapiens | 2 | ALA4270621 | assay format | Scientific Literature | |
11. | ALA4275256 | Binding | Displacement of [3H]CP55940 from recombinant human CB1 expressed in crude membranes at 10 uM after 90 mins by liquid scintillation counting method relative to control | Homo sapiens | 2 | ALA4270621 | cell membrane format | Scientific Literature | |
12. | ALA4275257 | Binding | Displacement of [3H]CP55940 from recombinant human CB2 expressed in crude membranes at 10 uM after 90 mins by liquid scintillation counting method relative to control | Homo sapiens | 2 | ALA4270621 | cell membrane format | Scientific Literature | |
13. | ALA4275258 | Binding | Selectivity factor, ratio of IC50 for FAAH in human U937 cells to IC50 for recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli | Homo sapiens | 2 | ALA4270621 | cell-based format | Scientific Literature | |
14. | ALA4275259 | Binding | Selectivity factor, ratio of IC50 for recombinant human ABHD6 expressed in HEK293 cells to IC50 for recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli | Homo sapiens | 2 | ALA4270621 | cell-based format | Scientific Literature | |
15. | ALA4275260 | Binding | Selectivity factor, ratio of IC50 for recombinant human ABHD12 expressed in HEK293 cells to IC50 for recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli | Homo sapiens | 2 | ALA4270621 | cell-based format | Scientific Literature | |
16. | ALA4275261 | Binding | Selectivity factor, ratio of Ki for displacement of [3H]CP55940 from recombinant human CB1 expressed in crude membranes expressed in HEK293 cells to IC50 for recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli | Homo sapiens | 2 | ALA4270621 | cell-based format | Scientific Literature | |
17. | ALA4275262 | Binding | Selectivity factor, ratio of Ki for displacement of [3H]CP55940 from recombinant human CB2 expressed in crude membranes expressed in HEK293 cells to IC50 for recombinant human C-terminal His-tagged MAGL expressed in Escherichia coli | Homo sapiens | 2 | ALA4270621 | cell-based format | Scientific Literature | |
18. | ALA4275263 | Binding | Displacement of [3H]-estradiol from full length human untagged ERalpha expressed in Sf insect cells after 18 to 24 hrs by radiometric method relative to estradiol | Homo sapiens | 3 | ALA4270621 | cell-based format | Scientific Literature | |
19. | ALA4275264 | Binding | Displacement of [3H]-estradiol from full length human ERbeta expressed in insect cells after 18 to 24 hrs by radiometric method relative to estradiol | Homo sapiens | 3 | ALA4270621 | cell-based format | Scientific Literature | |
20. | ALA4275265 | Functional | Antiproliferative activity against human MDA-MB-231 cells measured after 96 hrs | Homo sapiens | 3 | ALA4270621 | cell-based format | Scientific Literature |