| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4272239 | Binding | Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins | Mus musculus | 17 | ALA4270537 | single protein format | Scientific Literature | |
| 2. | ALA4272240 | Binding | Inhibition of recombinant human full length GST-tagged wild type BRAF expressed in baculovirus expression system using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins | Homo sapiens | 17 | ALA4270537 | assay format | Scientific Literature | |
| 3. | ALA4272241 | Functional | Antiproliferative activity against human A375 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 4. | ALA4272242 | Functional | Antiproliferative activity against human WM266.4 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 5. | ALA4272243 | Functional | Antiproliferative activity against human WM1361 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 6. | ALA4272244 | Functional | Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 7. | ALA4272245 | Functional | Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay | Homo sapiens | 17 | ALA4270537 | cell-based format | Scientific Literature | |
| 8. | ALA4272246 | Binding | Inhibition of recombinant human GST-tagged c-RAF (306 to 648 residues) Y340D/Y341D mutant expressed in baculovirus expression system using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins | Homo sapiens | 3 | ALA4270537 | assay format | Scientific Literature | |
| 9. | ALA4272247 | ADME | Cytotoxicity against HEK293T cells assessed as decrease in cell viability after 48 hrs by MTT assay | Homo sapiens | 3 | ALA4270537 | cell-based format | Scientific Literature | |
| 10. | ALA4272248 | ADME | Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 48 hrs by MTT assay | Homo sapiens | 3 | ALA4270537 | cell-based format | Scientific Literature | |
| 11. | ALA4272249 | Binding | Inhibition of MEK1/2 (unknown origin) | Homo sapiens | 3 | ALA4270537 | protein format | Scientific Literature | |
| 12. | ALA4272250 | Binding | Inhibition of N-terminal flag-tagged JNK1 (unknown origin) expressed in baculovirus expression system after 60 mins in presence of [gamma-32P]ATP by topcount scintillation method | Homo sapiens | 3 | ALA4270537 | assay format | Scientific Literature | |
| 13. | ALA4272251 | Binding | Inhibition of N-terminal flag-tagged p38alpha (unknown origin) expressed in baculovirus expression system using MBP as substrate preincubated for 5 mins followed by substrate addition measured after 60 mins in presence of [gamma-32P]ATP by topcount scintillation method | Homo sapiens | 3 | ALA4270537 | assay format | Scientific Literature | |
| 14. | ALA4272304 | Binding | Inhibition of BRAF V600E mutant in human A375 cells assessed as decrease in MEK1/2 phosphorylation up to 0.3 uM after 30 mins by Western blot analysis | Homo sapiens | 1 | ALA4270537 | cell-based format | Scientific Literature |
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