# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4271662 | B | Displacement of 1,8-ANS from FABP4 (unknown origin) after 3 mins by fluorescence assay | Homo sapiens | 37 | ALA4270520 | single protein format | Scientific Literature | |
2. | ALA4271663 | B | Binding affinity to FABP4 (unknown origin) by isothermal calorimetric titration method | Homo sapiens | 2 | ALA4270520 | single protein format | Scientific Literature | |
3. | ALA4271664 | A | Inhibition of FABP3 (unknown origin) | Homo sapiens | 6 | ALA4270520 | single protein format | Scientific Literature | |
4. | ALA4271665 | B | Selectivity ratio of IC50 for FABP3 (unknown origin) to IC50 for displacement of 1,8-ANS from FABP4 (unknown origin) | Homo sapiens | 6 | ALA4270520 | assay format | Scientific Literature | |
5. | ALA4271666 | F | Inhibition of recombinant human GPR40 expressed in CHO cells assessed as reduction in intracellular calcium level at 10 uM by Fluo-4AM dye based fluorescence assay | Homo sapiens | 2 | ALA4270520 | cell-based format | Scientific Literature | |
6. | ALA4271667 | F | Inhibition of recombinant human GPR120 expressed in CHO cells assessed as reduction in intracellular calcium level at 10 uM by Fluo-4AM dye based fluorescence assay | Homo sapiens | 2 | ALA4270520 | cell-based format | Scientific Literature | |
7. | ALA4271668 | B | Inhibition of recombinant human N-terminal His6/epitope-tagged DGAT1 expressed in baculovirus infected Sf9 cell membranes at 10 uM using didecanoyl glycerol/[1-14C]decanoyl-CoA as substrate measured after 60 mins by Topcount scintillation counting method | Homo sapiens | 2 | ALA4270520 | cell-based format | Scientific Literature | |
8. | ALA4271669 | B | Inhibition of recombinant PPARgamma (unknown origin) expressed in HEK293 cells at 10 uM after 24 hrs by Steady-Glo luciferase reporter gene assay | Homo sapiens | 2 | ALA4270520 | cell-based format | Scientific Literature | |
9. | ALA4271670 | A | Cytotoxicity against mouse 3T3L1 pre-adipocytes assessed as effect on cell viability up to 80 uM after 24 hrs by MTT assay | Mus musculus | 2 | ALA4270520 | cell-based format | Scientific Literature | |
10. | ALA4271672 | B | Inhibition of FABP4 in mouse 3T3L1 cells assessed as reduction in forskolin-stimulated lipolysis by measuring reduction in glycerol level pretreated for 24 hrs followed by forskolin stimulation and measured after 2 hrs | Mus musculus | 2 | ALA4270520 | cell-based format | Scientific Literature | |
11. | ALA4271673 | B | Inhibition of FABP4 in mouse 3T3L1 pre-adipocytes assessed as increase in triglyceride level at 50 uM treated for 6 days during differentiation period supplemented with fresh medium containing compounds for every 2 days measured post last dose | Mus musculus | 2 | ALA4270520 | cell-based format | Scientific Literature | |
12. | ALA4271674 | B | Inhibition of FABP4 in mouse 3T3L1 pre-adipocytes assessed as increase in lipid accumulation at 50 uM treated for 6 days during differentiation period supplemented with fresh medium containing compounds for every 2 days measured post last dose by Oil red O staining based microscopic analysis | Mus musculus | 2 | ALA4270520 | cell-based format | Scientific Literature | |
13. | ALA4271675 | A | Half life in human liver microsomes at 0.1 uM by HPLC-MS/MS analysis | Homo sapiens | 2 | ALA4270520 | microsome format | Scientific Literature | |
14. | ALA4271676 | A | Half life in rat liver microsomes at 0.1 uM by HPLC-MS/MS analysis | Rattus norvegicus | 2 | ALA4270520 | microsome format | Scientific Literature | |
15. | ALA4271677 | A | Half life in mouse liver microsomes at 0.1 uM by HPLC-MS/MS analysis | Mus musculus | 2 | ALA4270520 | microsome format | Scientific Literature | |
16. | ALA4271678 | A | Intrinsic clearance in human liver microsomes assessed per gram of protein at 0.1 uM by HPLC-MS/MS analysis | Homo sapiens | 2 | ALA4270520 | microsome format | Scientific Literature | |
17. | ALA4271679 | A | Intrinsic clearance in rat liver microsomes assessed per gram of protein at 0.1 uM by HPLC-MS/MS analysis | Rattus norvegicus | 2 | ALA4270520 | microsome format | Scientific Literature | |
18. | ALA4271680 | A | Intrinsic clearance in mouse liver microsomes assessed per gram of protein at 0.1 uM by HPLC-MS/MS analysis | Mus musculus | 2 | ALA4270520 | microsome format | Scientific Literature | |
19. | ALA4271681 | A | Stability in human liver microsomes assessed as parent compound remaining at 0.1 uM after 60 mins by HPLC-MS/MS analysis | Homo sapiens | 2 | ALA4270520 | microsome format | Scientific Literature | |
20. | ALA4271682 | A | Stability in rat liver microsomes assessed as parent compound remaining at 0.1 uM after 60 mins by HPLC-MS/MS analysis | Rattus norvegicus | 2 | ALA4270520 | microsome format | Scientific Literature |