# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4421217 | B | Competitive binding affinity to full-length mouse SOX18 expressed in Sf9 cells assessed as reduction in SOX1 8- DNA binding by measuring FP-bound fraction at 200 uM using FAM-labelled Prox1 -DNA element incubated for 5 mins in dark at room temperature, 10 mins at 37 degC and 30 mins at room temperature by fluorescence polarization assay relative to control | Mus musculus | 30 | ALA4420080 | cell-based format | Patent Bioactivity Data | |
2. | ALA4421218 | B | Competitive binding affinity to full-length mouse SOX18 expressed in Sf9 cells assessed as reduction in SOX1 8- DNA binding by measuring FP-bound fraction at 50 uM using FAM-labelled Prox1 -DNA element incubated for 5 mins in dark at room temperature, 10 mins at 37 degC and 30 mins at room temperature by fluorescence polarization assay relative to control | Mus musculus | 30 | ALA4420080 | cell-based format | Patent Bioactivity Data | |
3. | ALA4421219 | A | Cytotoxicity in HEK293 cells assessed as reduction in cell viability incubated for 24 hrs by Alamar blue dye based assay | Homo sapiens | 30 | ALA4420080 | cell-based format | Patent Bioactivity Data | |
4. | ALA4421220 | A | Cytotoxicity in human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by Alamar blue dye based assay | Homo sapiens | 30 | ALA4420080 | cell-based format | Patent Bioactivity Data |