#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4372582	Binding	Inhibition of USP9x in HEK293T cell lysates assessed as enzyme recovery at 250 uM using HA-tagged vinyl-sulfone as substrate preincubated for 40 mins followed by 10-fold dilution and subsequent substrate addition measured up to 2 hrs by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
2.	ALA4372588	Binding	Inhibition of deubiquitinase in HEK293T cells expressing N-terminal HA-tagged ubiquitin assessed as stabilization of HMW-Ub proteins after 2 hrs by Western blot analysis	Homo sapiens	24	ALA4371052	cell-based format	Scientific Literature
3.	ALA4372591	Binding	Inhibition of deubiquitinase in HEK293T cells assessed as stabilization of K48-linked HMW-Ub protein by Western blot analysis	Homo sapiens	4	ALA4371052	cell-based format	Scientific Literature
4.	ALA4372592	Binding	Inhibition of USP9x in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
5.	ALA4372593	Binding	Inhibition of USP19 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
6.	ALA4372594	Binding	Inhibition of USP7 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
7.	ALA4372595	Binding	Inhibition of USP8 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
8.	ALA4372596	Binding	Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
9.	ALA4372597	Binding	Inhibition of USP17 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
10.	ALA4372598	Binding	Inhibition of UCHL1 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
11.	ALA4372599	Binding	Inhibition of UCHL3 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
12.	ALA4372600	Binding	Inhibition of USP7 in HEK293T cell lysates assessed as ratio of HA-Ub-VS modified USP7 to total USP7 at 50 uM using HA-Ub vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis (Rvb = 0.5 No_unit)	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
13.	ALA4372601	Binding	Inhibition of USP7 in HEK293T cell lysates assessed as ratio of HA-Ub-VS modified USP7 to total USP7 at 100 uM using HA-Ub vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis (Rvb = 0.5 No_unit)	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
14.	ALA4372602	Binding	Inhibition of USP9x in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
15.	ALA4372603	Binding	Inhibition of USP7 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
16.	ALA4372604	Binding	Inhibition of USP8 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
17.	ALA4372605	Binding	Inhibition of USP19 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
18.	ALA4372606	Binding	Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
19.	ALA4372607	Binding	Inhibition of USP15 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
20.	ALA4372608	Binding	Inhibition of UCHL5 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature