| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4372582 | B | Inhibition of USP9x in HEK293T cell lysates assessed as enzyme recovery at 250 uM using HA-tagged vinyl-sulfone as substrate preincubated for 40 mins followed by 10-fold dilution and subsequent substrate addition measured up to 2 hrs by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 2. | ALA4372588 | B | Inhibition of deubiquitinase in HEK293T cells expressing N-terminal HA-tagged ubiquitin assessed as stabilization of HMW-Ub proteins after 2 hrs by Western blot analysis | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
| 3. | ALA4372591 | B | Inhibition of deubiquitinase in HEK293T cells assessed as stabilization of K48-linked HMW-Ub protein by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 4. | ALA4372592 | B | Inhibition of USP9x in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 5. | ALA4372593 | B | Inhibition of USP19 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 6. | ALA4372594 | B | Inhibition of USP7 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 7. | ALA4372595 | B | Inhibition of USP8 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 8. | ALA4372596 | B | Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 9. | ALA4372597 | B | Inhibition of USP17 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 10. | ALA4372598 | B | Inhibition of UCHL1 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 11. | ALA4372599 | B | Inhibition of UCHL3 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 12. | ALA4372600 | B | Inhibition of USP7 in HEK293T cell lysates assessed as ratio of HA-Ub-VS modified USP7 to total USP7 at 50 uM using HA-Ub vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis (Rvb = 0.5 No_unit) | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 13. | ALA4372601 | B | Inhibition of USP7 in HEK293T cell lysates assessed as ratio of HA-Ub-VS modified USP7 to total USP7 at 100 uM using HA-Ub vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis (Rvb = 0.5 No_unit) | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 14. | ALA4372602 | B | Inhibition of USP9x in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 15. | ALA4372603 | B | Inhibition of USP7 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 16. | ALA4372604 | B | Inhibition of USP8 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 17. | ALA4372605 | B | Inhibition of USP19 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 18. | ALA4372606 | B | Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 19. | ALA4372607 | B | Inhibition of USP15 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 20. | ALA4372608 | B | Inhibition of UCHL5 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature |
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