#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4407308	Functional	Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric affinity cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	67	ALA4406806	cell-based format	Scientific Literature
2.	ALA4407309	Functional	Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring efficacy cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	67	ALA4406806	cell-based format	Scientific Literature
3.	ALA4407310	Functional	Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric binding affinity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	67	ALA4406806	cell-based format	Scientific Literature
4.	ALA4407311	Functional	Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric index in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	67	ALA4406806	cell-based format	Scientific Literature
5.	ALA4407313	Functional	Positive allosteric modulation of human GPR4 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production at higher concentration in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	4	ALA4406806	cell-based format	Scientific Literature
6.	ALA4407314	Functional	Positive allosteric modulation of human GPR65 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production at higher concentration in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	4	ALA4406806	cell-based format	Scientific Literature
7.	ALA4407316	Binding	Displacement of [3H]ZM241385 from recombinant human A2A receptor incubated for 90 mins under dark condition by microbeta scintillation method	Homo sapiens	1	ALA4406806	single protein format	Scientific Literature
8.	ALA4407319	Functional	Agonist activity at recombinant human A2A receptor expressed in HEK293T cells co-expressing Gs assessed as stimulation of cAMP accumulation measured after 15 mins by Glosensor chemiluminescence assay	Homo sapiens	1	ALA4406806	cell-based format	Scientific Literature
9.	ALA4407320	Functional	Inverse agonist activity at recombinant human A2A receptor expressed in HEK293T cells co-expressing Gs assessed as decrease in cAMP accumulation measured after 15 mins by Glosensor chemiluminescence assay	Homo sapiens	1	ALA4406806	cell-based format	Scientific Literature
10.	ALA4407321	Functional	Antagonist activity at recombinant human A2A receptor expressed in HEK293T cells co-expressing Gs assessed as inhibition of NECA-stimulated cAMP accumulation preincubated for 15 mins followed by NECA addition and measured after 15 mins by Glosensor chemiluminescence assay	Homo sapiens	1	ALA4406806	cell-based format	Scientific Literature
11.	ALA4407324	Binding	Agonist activity at human ACKR3 expressed in HTLA cells assessed as stimulation of beta-arrestin recruitment at 1 uM incubated for 16 to 20 hrs by bright-glo luminescence assay	Homo sapiens	3	ALA4406806	cell-based format	Scientific Literature
12.	ALA4407329	Functional	Positive allosteric modulation of mouse GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric affinity cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Mus musculus	6	ALA4406806	cell-based format	Scientific Literature
13.	ALA4407330	Functional	Positive allosteric modulation of mouse GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring efficacy cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Mus musculus	6	ALA4406806	cell-based format	Scientific Literature
14.	ALA4407331	Functional	Positive allosteric modulation of mouse GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric binding affinity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Mus musculus	6	ALA4406806	cell-based format	Scientific Literature
15.	ALA4407332	Functional	Positive allosteric modulation of mouse GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric index in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Mus musculus	6	ALA4406806	cell-based format	Scientific Literature