#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4356123	B	Inhibition of recombinant human IDO1 expressed in Escherichia coli BL21 cells assessed as reduction in conversion of L-tryptophan to N-formyl kynurenine using L-tryptophan as substrate after 30 mins by methylene blue dye based microplate reader analysis	Homo sapiens	14	ALA4354812	cell-based format	Scientific Literature
2.	ALA4356124	B	Inhibition of C-terminal His6-tagged human IDO2 (14 to 420 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in conversion of L-tryptophan to N-formyl kynurenine using L-tryptophan as substrate after 30 mins by methylene blue dye based microplate reader analysis	Homo sapiens	13	ALA4354812	assay format	Scientific Literature
3.	ALA4356125	B	Inhibition of recombinant human TDO expressed in Escherichia coli BL21 (DE3) assessed as reduction in conversion of L-tryptophan to N-formyl kynurenine using L-tryptophan as substrate after 30 mins by methylene blue assay	Homo sapiens	14	ALA4354812	assay format	Scientific Literature
4.	ALA4356126	B	Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells assessed as reduction in kynurenine production incubated for 18 hrs	Homo sapiens	13	ALA4354812	cell-based format	Scientific Literature
5.	ALA4356127	B	Inhibition of human IDO2 transfected in human U87MG cells assessed as reduction in kynurenine production using L-tryptophan as substrate incubated with for 6 hrs	Homo sapiens	13	ALA4354812	cell-based format	Scientific Literature
6.	ALA4356128	B	Inhibition of human TDO transfected in HEK293 cells assessed as reduction in kynurenine production using L-tryptophan as substrate incubated for 12 hrs	Homo sapiens	14	ALA4354812	cell-based format	Scientific Literature
7.	ALA4356130	B	Uncompetitive inhibition of recombinant human IDO1 expressed in Escherichia coli BL21 cells assessed inhibition constant using varying concentration of L-tryptophan as substrate by Dixon plot analysis	Homo sapiens	11	ALA4354812	cell-based format	Scientific Literature
8.	ALA4356132	B	Mixed competitive inhibition of C-terminal His6-tagged human IDO2 (14 to 420 residues) expressed in Escherichia coli BL21 (DE3) assessed as inhibition constant using varying concentration of L-tryptophan as substrate by Dixon plot analysis	Homo sapiens	2	ALA4354812	assay format	Scientific Literature
9.	ALA4356135	B	Uncompetitive inhibition of recombinant human TDO expressed in Escherichia coli BL21 (DE3) assessed as assessed as inhibition constant using varying concentration of L-tryptophan as substrate by Dixon plot analysis	Homo sapiens	9	ALA4354812	assay format	Scientific Literature
10.	ALA4356138	B	Binding affinity to human IDO1 assessed as change in spectral signal at 200 uM by CPMG NMR spectrometry analysis	Homo sapiens	2	ALA4354812	single protein format	Scientific Literature
11.	ALA4356139	B	Binding affinity to human IDO2 assessed as change in spectral signal at 200 uM by CPMG NMR spectrometry analysis	Homo sapiens	2	ALA4354812	single protein format	Scientific Literature
12.	ALA4356140	B	Binding affinity to human TDO assessed as change in spectral signal at 200 uM by CPMG NMR spectrometry analysis	Homo sapiens	2	ALA4354812	single protein format	Scientific Literature
13.	ALA4356141	B	Binding affinity to human IDO1 at 200 uM by STD NMR spectrometry analysis	Homo sapiens	2	ALA4354812	single protein format	Scientific Literature
14.	ALA4356142	B	Binding affinity to human IDO2 at 200 uM by STD NMR spectrometry analysis	Homo sapiens	2	ALA4354812	single protein format	Scientific Literature
15.	ALA4356143	B	Binding affinity to human TDO at 200 uM by STD NMR spectrometry analysis	Homo sapiens	2	ALA4354812	single protein format	Scientific Literature
16.	ALA4356144	B	Binding affinity to heme in human IDO1 assessed as Soret band red shift from 405 nm to 410 nM at 375 uM and pH 6.5 under normal atmospheric condition by UV-vis spectroscopy analysis	Homo sapiens	2	ALA4354812	single protein format	Scientific Literature
17.	ALA4356145	B	Inhibition of IDO1/TDO in IFNgamma-treated human HeLa cells assessed as T cell proliferation at 1 uM measured after 2 days in presence of IL-2, anti-CD3 and IFN-gamma by CFSE dye based flow cytometry analysis (Rvb = 11.7 %)	Homo sapiens	2	ALA4354812	assay format	Scientific Literature
18.	ALA4356146	B	Inhibition of IDO1/TDO in IFNgamma-treated human HeLa cells assessed as T cell proliferation at 40 nM measured after 2 days in presence of IL-2, anti-CD3 and IFN-gamma by CFSE dye based flow cytometry analysis (Rvb = 11.7 %)	Homo sapiens	1	ALA4354812	assay format	Scientific Literature
19.	ALA4356147	B	Inhibition of IDO1/TDO in IFNgamma-treated human HeLa cells assessed as T cell proliferation at 200 nM measured after 2 days in presence of IL-2, anti-CD3 and IFN-gamma by CFSE dye based flow cytometry analysis (Rvb = 11.7 %)	Homo sapiens	1	ALA4354812	assay format	Scientific Literature
20.	ALA4356148	B	Inhibition of IDO1/TDO in IFNgamma-treated human HeLa cells assessed as T cell proliferation at 500 nM measured after 2 days in presence of IL-2, anti-CD3 and IFN-gamma by CFSE dye based flow cytometry analysis (Rvb = 11.7 %)	Homo sapiens	1	ALA4354812	assay format	Scientific Literature