# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4353043 | Binding | Inhibition of dengue virus serotype 2 His-tagged NS2B cofactor domain-NS3 protease expressed in Escherichia coli at 50 uM using 2-Abz-Nle-Lys-Arg-Arg-Ser-(3-NO2)-Tyr-NH2 as substrate preincubated with enzyme for 15 mins followed by substrate addition measured for every 1 sec for 15 mins by fluorescence plate reader based HPLC analysis relative to control | dengue virus type 2 | 13 | ALA4350980 | assay format | Scientific Literature | |
2. | ALA4353044 | Binding | Inhibition of West Nile virus His-tagged NS2B cofactor domain-NS3 protease expressed in Escherichia coli at 50 uM using 2-Abz-Gly-Leu-Lys-Arg-Gly-Gly-(3-NO2)-Tyr-NH2 as substrate preincubated with enzyme for 15 mins followed by substrate addition measured for every 1 sec for 15 mins by fluorescence microplate reader based HPLC analysis relative to control | West Nile virus | 13 | ALA4350980 | assay format | Scientific Literature | |
3. | ALA4353045 | Binding | Inhibition of thrombin (unknown origin) at 25 uM using Boc-Val-Pro-Arg-AMC as substrate preincubated with enzyme for 15 mins followed by addition of substrate by fluorescence plate reader analysis relative to control | Homo sapiens | 13 | ALA4350980 | single protein format | Scientific Literature | |
4. | ALA4353046 | Binding | Inhibition of trypsin (unknown origin) at 50 uM using Boc-Val-Pro-Arg-AMC as substrate preincubated with enzyme for 15 mins followed by addition of substrate by fluorescence plate reader analysis relative to control | Homo sapiens | 13 | ALA4350980 | protein format | Scientific Literature | |
5. | ALA4353049 | Binding | Inhibition of trypsin (unknown origin) using Boc-Val-Pro-Arg-AMC as substrate preincubated with enzyme for 15 mins followed by addition of substrate by fluorescence plate reader analysis | Homo sapiens | 9 | ALA4350980 | protein format | Scientific Literature | |
6. | ALA4353060 | Binding | Inhibition of dengue virus serotype 2 His-tagged NS2B cofactor domain-NS3 protease expressed in Escherichia coli at 1 mM using 2-Abz-Nle-Lys-Arg-Arg-Ser-(3-NO2)-Tyr-NH2 as substrate preincubated with enzyme for 15 mins followed by substrate addition measured for every 1 sec for 15 mins by fluorescence plate reader based HPLC analysis relative to control | dengue virus type 2 | 9 | ALA4350980 | assay format | Scientific Literature | |
7. | ALA4353061 | Binding | Inhibition of West Nile virus His-tagged NS2B cofactor domain-NS3 protease expressed in Escherichia coli at 1 mM using 2-Abz-Gly-Leu-Lys-Arg-Gly-Gly-(3-NO2)-Tyr-NH2 as substrate preincubated with enzyme for 15 mins followed by substrate addition measured for every 1 sec for 15 mins by fluorescence microplate reader based HPLC analysis relative to control | West Nile virus | 9 | ALA4350980 | assay format | Scientific Literature | |
8. | ALA4353062 | Binding | Inhibition of thrombin (unknown origin) at 500 uM using Boc-Val-Pro-Arg-AMC as substrate preincubated with enzyme for 15 mins followed by addition of substrate by fluorescence plate reader analysis relative to control | Homo sapiens | 9 | ALA4350980 | single protein format | Scientific Literature | |
9. | ALA4353063 | Binding | Inhibition of trypsin (unknown origin) at 1 mM using Boc-Val-Pro-Arg-AMC as substrate preincubated with enzyme for 15 mins followed by addition of substrate by fluorescence plate reader analysis relative to control | Homo sapiens | 9 | ALA4350980 | protein format | Scientific Literature | |
10. | ALA4353064 | Binding | Time-dependent inhibition of dengue virus serotype 2 His-tagged NS2B cofactor domain-NS3 protease expressed in Escherichia coli at 200 uM using 2-Abz-Nle-Lys-Arg-Arg-Ser-(3-NO2)-Tyr-NH2 as substrate preincubated with enzyme for 20 to 40 mins followed by substrate addition measured for every 1 sec for 15 mins by fluorescence plate reader based HPLC analysis | dengue virus type 2 | 1 | ALA4350980 | assay format | Scientific Literature | |
11. | ALA4353068 | Binding | Reversible covalent binding affinity at dengue virus serotype 2 His-tagged NS2B cofactor domain-NS3 protease expressed in Escherichia coli assessed as acyl-enzyme adduct formation at 100 uM incubated for 5 to 24 hrs by ESI-MS analysis | dengue virus type 2 | 2 | ALA4350980 | assay format | Scientific Literature |