| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4432140 | A | Cytotoxicity in RFC-null Chinese hamster R2 cells assessed as reduction in cell viability measured after 96 hrs by Cell-Titer Blue fluorescence analysis | Cricetulus griseus | 10 | ALA4431326 | cell-based format | Scientific Literature | |
| 2. | ALA4432141 | B | Inhibition of human RFC expressed in Chinese hamster PC43-10 cells assessed as antiproliferative activity measured as reduction in cell viability after 96 hrs by Cell-Titer Blue fluorescence analysis | Homo sapiens | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 3. | ALA4432142 | B | Inhibition of human FR-alpha receptor expressed in Chinese hamster RT16 cells assessed as antiproliferative activity measured as reduction in cell viability after 96 hrs by Cell-Titer Blue fluorescence analysis | Homo sapiens | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 4. | ALA4432143 | B | Inhibition of human FR-beta receptor expressed in Chinese hamster D4 cells assessed as antiproliferative activity measured as reduction in cell viability after 96 hrs by Cell-Titer Blue fluorescence analysis | Homo sapiens | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 5. | ALA4432144 | B | Inhibition of human PCFT expressed in Chinese hamster R2/PCFT4 cells assessed as antiproliferative activity measured as reduction in cell viability after 96 hrs by Cell-Titer Blue fluorescence analysis | Homo sapiens | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 6. | ALA4432145 | A | Cytotoxicity in PCFT-null Chinese hamster R2 cells assessed as reduction in cell viability measured after 96 hrs by Cell-Titer Blue fluorescence analysis | Cricetulus griseus | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 7. | ALA4432146 | F | Antiproliferative activity against human KB cells expressing human RFC/FR-alpha/PCFT assessed as reduction in cell viability measured after 96 hrs by Cell-Titer Blue fluorescence analysis | Homo sapiens | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 8. | ALA4432147 | F | Antiproliferative activity against human KB cells expressing human RFC/FR-alpha/PCFT assessed as reduction in cell viability measured after 96 hrs in presence of folic acid by Cell-Titer Blue fluorescence analysis | Homo sapiens | 7 | ALA4431326 | cell-based format | Scientific Literature | |
| 9. | ALA4432148 | B | Inhibition of GARFTase/AICARFTase in human KB cells expressing RFC/FR-alpha/PCFT assessed as cell growth inhibition measured after 96 hrs in presence of 60 uM adenosine by Cell-Titer Blue fluorescence analysis | Homo sapiens | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 10. | ALA4432149 | B | Inhibition of GARFTase/AICARFTase in human KB cells expressing RFC/FR-alpha/PCFT assessed as cell growth inhibition measured after 96 hrs in presence of 10 uM thymidine by Cell-Titer Blue fluorescence analysis | Homo sapiens | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 11. | ALA4432150 | B | Inhibition of AICARFTase in human KB cells expressing RFC/FR-alpha/PCFT assessed as cell growth inhibition measured after 96 hrs in presence of 320 uM AICA by Cell-Titer Blue fluorescence analysis | Homo sapiens | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 12. | ALA4432151 | B | Displacement of [3H]folic acid from human FR-alpha receptor expressed in Chinese hamster RT16 cells measured after 15 mins relative to folic acid | Homo sapiens | 6 | ALA4431326 | cell-based format | Scientific Literature | |
| 13. | ALA4432152 | B | Displacement of [3H]folic acid from human FR-beta receptor expressed in Chinese hamster D4 cells measured after 15 mins relative to folic acid | Homo sapiens | 3 | ALA4431326 | cell-based format | Scientific Literature | |
| 14. | ALA4432154 | B | Inhibition of [3H]MTX uptake at human PCFT expressed in Chinese hamster R2/PCFT4 cells at 10 uM and pH 5.5 measured after 5 mins | Homo sapiens | 7 | ALA4431326 | cell-based format | Scientific Literature | |
| 15. | ALA4432155 | B | Inhibition of [3H]MTX uptake at human PCFT expressed in Chinese hamster R2/PCFT4 cells at 10 uM and pH 6.8 measured after 5 mins | Homo sapiens | 7 | ALA4431326 | cell-based format | Scientific Literature | |
| 16. | ALA4432156 | B | Inhibition of [3H]MTX uptake at human PCFT expressed in Chinese hamster R2/PCFT4 cells at 1 uM and pH 5.5 measured after 5 mins | Homo sapiens | 7 | ALA4431326 | cell-based format | Scientific Literature | |
| 17. | ALA4432157 | B | Inhibition of [3H]MTX uptake at human PCFT expressed in Chinese hamster R2/PCFT4 cells at pH 5.5 measured after 5 mins by Dixon plot analysis | Homo sapiens | 8 | ALA4431326 | cell-based format | Scientific Literature | |
| 18. | ALA4432158 | B | Inhibition of GARFTase in human KB cells expressing RFC/FR-alpha/PCFT assessed as decrease in incorporation of [14C(U)]-glycine into [14C]formyl GAR incubated for 1 hr followed by addition of [14C(U)]-glycine measured after 16 hrs in presence of azaserine | Homo sapiens | 7 | ALA4431326 | cell-based format | Scientific Literature | |
| 19. | ALA4432159 | B | Inhibition of recombinant N-terminal 6His-tagged human GARFTase assessed as formation of 5,8-dideazafolate from 10-formyl-5,8-dideazafolic acid measured at every 15 seconds over 20 min by spectrophotometric analysis | Homo sapiens | 9 | ALA4431326 | single protein format | Scientific Literature | |
| 20. | ALA4432160 | B | Potency index, ratio of PMX Ki to test compound Ki for recombinant N-terminal 6His-tagged human GARFTase | Homo sapiens | 8 | ALA4431326 | single protein format | Scientific Literature |
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