| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4329767 | B | Inhibition of Cy5-preE-let-7g binding to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS measured after 30 to 60 mins by electrophoretic mobility shift assay | Homo sapiens | 2 | ALA4325922 | assay format | Scientific Literature | |
| 2. | ALA4329769 | B | Inhibition of Cy5-preE-let-7a-1 binding to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS measured after 30 to 60 mins by electrophoretic mobility shift assay | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 3. | ALA4329770 | B | Inhibition of PreE-let-7a-1-BHQ2 binding to recombinant human N-terminal His6-tagged/Cy3-labelled Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS measured after 30 to 60 mins by FRET assay | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 4. | ALA4329771 | B | Inhibition of pre-let-7g binding to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS assessed as increase in dicer-mediated pre-let-7g microRNA maturation at 5 to 10 uM measured after 40 mins by SYBR green2 dye based fluorescence assay | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 5. | ALA4329772 | B | Inhibition of recombinant FLAG-tagged human dicer expressed in HEK293 cells assessed as reduction in pre-let-7g microRNA maturation at 5 to 10 uM after 40 mins by SYBR green2 dye based fluorescence assay | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 6. | ALA4329773 | B | Binding affinity to recombinant human N-terminal His6-tagged Lin28a cold shock domain expressed in Escherichia coli Rosetta2 (DE3) pLysS at 0.625 to 10 uM by surface plasmon resonance analysis | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 7. | ALA4329774 | B | Binding affinity to recombinant human N-terminal His6-tagged Lin28a zinc knuckle domain expressed in Escherichia coli Rosetta2 (DE3) pLysS at 0.625 to 10 uM by surface plasmon resonance analysis | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 8. | ALA4329775 | B | Inhibition of biotinylated-preE-let-7g binding to Lin28b in human JAR cells by pull down assay | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 9. | ALA4329776 | B | Inhibition of biotinylated-preE-let-7g binding to Lin28a in human JAR cells by pull down assay | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 10. | ALA4329777 | B | Binding affinity to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS by surface plasmon resonance analysis | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 11. | ALA4329778 | B | Inhibition of biotinylated-preE-let-7g binding to hnRNPA1 in human JAR cells by pull down assay | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 12. | ALA4329779 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as increase in mature let-7a level after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 13. | ALA4329780 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as increase in mature let-7b level after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 14. | ALA4329781 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as increase in mature let-7d level after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 15. | ALA4329782 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as increase in mature let-7f level after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 16. | ALA4329783 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as increase in mature let-7g level after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 17. | ALA4329784 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as increase in mature let-7i level after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 18. | ALA4329785 | F | Effect on miR-98 level in human JAR cells at 5 to 10 uM after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 19. | ALA4329786 | F | Effect on miR-20b level in human JAR cells at 5 to 10 uM after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 20. | ALA4329789 | F | Increase in mature let-7d level in Lin28a/b knockdown human JAR cells at 10 uM after 20 hrs by qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature |
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