# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4329768 | B | Inhibition of Cy5-preE-let-7g binding to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS at 15 uM measured after 30 to 60 mins by electrophoretic mobility shift assay relative to control | Homo sapiens | 2 | ALA4325922 | assay format | Scientific Literature | |
2. | ALA4329787 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as increase in mature let-7a level at 5 to 10 uM after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
3. | ALA4329788 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as increase in mature let-7g level at 5 to 10 uM after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
4. | ALA4329802 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as reduction in HMGA2 expression at 5 to 10 uM after 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA4325922 | cell-based format | Scientific Literature | |
5. | ALA4329803 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as reduction in IMP1 expression at 5 to 10 uM after 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA4325922 | cell-based format | Scientific Literature | |
6. | ALA4329804 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as reduction in Ras expression at 5 to 10 uM after 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA4325922 | cell-based format | Scientific Literature | |
7. | ALA4329805 | B | Inhibition of Lin28a/Lin28b in human JAR cells assessed as reduction in Lin28a expression at 5 to 10 uM after 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA4325922 | cell-based format | Scientific Literature |